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Plant O-methyltransferases: molecular analysis, common signature and classification
Comparative analysis of the predicted amino acid sequences of a number of plant O-methyltransferase cDNA clones show that they share some 32–71% sequence identity, and can be grouped according to theExpand
Structure, function, and evolution of plant O-methyltransferases.
Plant O-methyltransferases (OMTs) constitute a large family of enzymes that methylate the oxygen atom of a variety of secondary metabolites including phenylpropanoids, flavonoids, and alkaloids.Expand
Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots
An abundant 17 kDa protein which was isolated and characterized from 10-day old healthy root tissue of white lupin (Lupinus albus) proved to have a high sequence similarity to pathogenesis-relatedExpand
Functional expression of an Arabidopsis cDNA clone encoding a flavonol 3'-O-methyltransferase and characterization of the gene product.
We report that the cDNA clone (Accession No. U70424), previously isolated from Arabidopsis thaliana as encoding a caffeic acid/5-hydroxyferulic acid O-methyltransferase (OMT) (1), has now beenExpand
Sequential O-methylation of tricetin by a single gene product in wheat.
Flavonoid compounds are ubiquitous in nature. They constitute an important part of the human diet and act as active principles of many medicinal plants. Their O-methylation increases theirExpand
Enzymology and compartmentation of polymethylated flavonol glucosides in chrysosplenium americanum
Abstract The enzymatic synthesis of polymethylated flavonol glucosides in Chrysosplenium americanum is catalysed by a number of distinct, position-specific O -methyltransferases and OExpand
Molecular and Biochemical Characterization of a Cold-Regulated PhosphoethanolamineN-Methyltransferase from Wheat1
A cDNA that encodes a methyltransferase (MT) was cloned from a cold-acclimated wheat (Triticum aestivum) cDNA library. Molecular analysis indicated that the enzyme WPEAMT (wheat phosphoethanolamineExpand
Aldonic Acids: A Novel Family of nod Gene Inducers of Mesorhizobium loti, Rhizobium lupini, and Sinorhizobium meliloti
Molecular signals, such as flavonoids (or nonflavonoid type), nod gene-inducers, and bacterial lipochitin oligosac-charides (LCOs) act as modulators of species specificity during early stages ofExpand
Enzymatic assay for flavonoid sulfotransferase.
A novel enzyme assay for flavonoid sulfotransferase is described. It makes use of tetrabutylammonium dihydrogen phosphate which forms a pair of ions with the flavonoid sulfate esters formed. ThisExpand
Novel flavonol 3-sulfotransferase. Purification, kinetic properties, and partial amino acid sequence.
A flavonol sulfotransferase (EC 2.8.2.-), which catalyzes the transfer of the sulfate group from 3'-phosphoadenosine 5'-phosphosulfate to the 3-hydroxyl group of flavonol aglycones, has been purifiedExpand