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Plant O-methyltransferases: molecular analysis, common signature and classification
TLDR
Cladistic analysis of the amino acid sequences suggests that plant O-methyltransferases may have arisen from common ancestral genes that were driven by different structural and/or functional requirements, and whose descendants segregated into different biochemical species.
Structure, function, and evolution of plant O-methyltransferases.
TLDR
A comparative and phylogenetic analysis of 61 biochemically characterized plant OMT protein sequences suggests that some OMTs may have undergone convergent evolution, while others show divergent evolution.
Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots
TLDR
Evidence is presented that the white lupin 17 kDa protein is constitutively expressed during all stages of root development and, to a lesser extent, in other plant parts.
Functional expression of an Arabidopsis cDNA clone encoding a flavonol 3'-O-methyltransferase and characterization of the gene product.
TLDR
The data indicate that this novel OMT uses the flavonol quercetin as the preferred substrate, but neither of the hydroxycinnamic acids, caffeic or 5-hydroxyferulic, to any significant extent, which indicates that the high sequence similarity/identity of AtOMT1 to that of the aspen lignin OMT is not sufficient to assign the function of this gene product.
Molecular and Biochemical Characterization of a Cold-Regulated PhosphoethanolamineN-Methyltransferase from Wheat1
TLDR
Northern and western analyses demonstrated that both Wpeamt transcript and the corresponding protein are up-regulated during cold acclimation, suggesting that the higher activity is due to de novo protein synthesis.
Enzymatic assay for flavonoid sulfotransferase.
Aldonic Acids: A Novel Family of nod Gene Inducers of Mesorhizobium loti, Rhizobium lupini, and Sinorhizobium meliloti
TLDR
The results indicate that both erythronic and tetronic acids led to low but significant increases in β-galactosidase activities, compared with the controls, and lupi-wighteone, a monoprenylated isoflavone, exerts a synergistic effect.
Molecular characterization and functional expression of flavonol 6-hydroxylase
TLDR
Recombinant F6H has been functionally expressed and characterized at the molecular level and the results demonstrate that its cofactor dependence, physicochemical characteristics and substrate preference compare well with the native enzyme.
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