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Alterations in Dihydropteroate Synthetase in Cell-Free Extracts of Sulfanilamide-Resistant Neisseria meningitidis and Neisseria gonorrhoeae
A direct relationship was observed between the minimal inhibitory concentration of sulfanilamide determined in vitro and the ratio of Ki/Km, which may be a molecular explanation of sulfAnilamide resistance for both N. meningitidis and N. gonorrhoeae. Expand
Structure-Activity of Sulfones and Sulfonamides on Dihydropteroate Synthetase from Neisseria meningitidis
The molecular interaction of various sulfones and sulfonamides with partially purified dihydropteroate synthetase from Neisseria meningitidis M-166 has been examined and 4,4′-Diaminodiphenylsulfone was three times more effective than sulfadiazine and nine times moreeffective than sulfanilamide as a competitive inhibitor of dihydopteroatesynthetase. Expand
A simple radioassay for dihydrofolate synthetase activity in Escherichia coli and its application to an inhibition study of new pteroate analogs.
A simple, rapid, and accurate radioassay method, using L-[ 14 C]glutamic acid as substrate, is developed for a quantitative determination of dihydrofolate synthetase activity in Escherichia coli. Expand
Characterization and inhibition of dihydrofolate synthetase from Neisseria gonorrhoeae
Dihydrofolate synthetase (EC 6.3.12) from N. gonorrhoeae was isolated and enzyme characteristics were determined, and patterns of product inhibition by dihydrofolates were found to be non-competitive with respect to dihydropteroate, and competitive withrespect to L-glutamate. Expand
Synthesis and biological evaluation of 2-amino-4-hydroxy-6-hydroxymethylpteridine pyrophosphate.
  • R. I. Ho
  • Chemistry, Medicine
  • Methods in enzymology
  • 1 September 1974
Abstract A method for synthesizing the title compound is described which represents a distinct improvement over previous procedures in regard to convenience, yield, and purity. IR and proton NMRExpand
Comparison of Dihydrofolate Reductases from Trimethoprim- and Sulfonamide‐resistant Strains of Neisseria gonorrhoeae
It was concluded that permeability of the cell envelope or increased production of dihydrofolate reductase may be an important factor among strains of N. gonorrhoeae that have low- and middle-range resistance to trimethoprim. Expand