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A closed tube format for amplification and detection of DNA based on energy transfer.
A new method for the direct detection of PCR-amplified DNA in a closed system is described. The method is based on the incorporation of energy transfer-labeled primers into the amplification product.Expand
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The receptor with high affinity for immunoglobulin E.
This review is about one of the physiologically important macromolecules that interact with the Fc domains of immunoglobulins. Such substances (complement, "immunoglobulin binding factors," FcExpand
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Immunophilin ligands demonstrate common features of signal transduction leading to exocytosis or transcription.
Investigations of the actions and interactions of the immunophilin ligands FK506, cyclosporin A (CsA), rapamycin, and 506BD suggest that complexes of FK506 with an FK506-binding protein or of CsAExpand
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Phospholipase C-gamma 1 is translocated to the membrane of rat basophilic leukemia cells in response to aggregation of IgE receptors.
Aggregation of the high affinity receptor for IgE (Fc epsilon RI) on the surface of mast cells results in the rapid hydrolysis of membrane inositol phospholipids by phospholipase C (PLC). Although atExpand
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Detection of telomerase activity utilizing energy transfer primers: comparison with gel- and ELISA-based detection.
We have developed a closed-tube format telomeric repeat amplification protocol (TRAP) assay for direct quantification of telomerase activity within the PCR vessel. The assay utilizes energy transferExpand
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Quantitative relationships between aggregation of IgE receptors, generation of intracellular signals, and histamine secretion in rat basophilic leukemia (2H3) cells. Enhanced responses with heavy
RBL-2H3 cells (a tumor analog of rat mast cells) have plasma-membrane receptors that bind immunoglobulin E, which when aggregated, initiate degranulation. As in other systems, secretion is preceededExpand
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Substance P, calcitonin gene-related peptide, and vasoactive intestinal peptide increase in nasal secretions after allergen challenge in atopic patients.
BACKGROUND There is suggestive evidence that neuropeptides participate in allergic reactions. Substance P (SP) and calcitonin gene-related peptide (CGRP) are released by sensory nerves, whereasExpand
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Distribution of secretory leukoprotease inhibitor in the human nasal airway.
Secretory leukoprotease inhibitor (SLPI) is a secreted glandular protein thought to regulate elastase activity and, more recently, to inhibit both mast cell chymase activity and histamine releaseExpand
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ERBIN deficiency links STAT3 and TGF-β pathway defects with atopy in humans
Nonimmunological connective tissue phenotypes in humans are common among some congenital and acquired allergic diseases. Several of these congenital disorders have been associated with eitherExpand
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S‐Adenosyl‐L‐homocysteine hydrolase from Dictyostelium discoideum is inactivated by cAMP and reactivated by NAD+
Purified S‐adenosyl‐L‐homocysteine hydrolase from Dictyostelium discoideum is inactivated when incubated at 25°C with cAMP. Half maximal velocity of the inactivation process occurs at 10 μM cAMP.Expand
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