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Cloning and sequence analysis of an Escherichia coli gene conferring bicyclomycin resistance.
TLDR
Cloned and sequenced DNA from Escherichia coli that, when present in a high-copy-number plasmid, confers resistance to the diketopiperazine antibiotic, bicyclomycin, contains the BcR gene, which had homology with the N-terminal regions of the CmR and TcR-encoded proteins and weak N- Terminals with the arabinose-proton symport protein. Expand
The biosynthesis of plant alkaloids and nitrogenous microbial metabolites.
  • R. Herbert
  • Chemistry, Medicine
  • Natural product reports
  • 1987
TLDR
This chapter reviews the results of investigations into the biosynthesis of alkaloids as well as those of, the quite often related, microbial metabolites containing nitrogen. Expand
Pigments of Pseudomonas species. IV. In vitro and in vivo conversion of 5-methylphenazinium-1-carboxylate into aeruginosin A.
5-Methylphenazinium-1-carboxylate has been synthesised. It has been converted by aqueous ammonia and cultures of Ps. aeruginosainto aeruginosin A.
Determinants of ligand binding affinity and cooperativity at the GLUT1 endofacial site.
Cytochalasin B (CB) and forskolin (FSK) inhibit GLUT1-mediated sugar transport in red cells by binding at or close to the GLUT1 endofacial sugar binding site. Paradoxically, very low concentrationsExpand
Microbial drug efflux proteins of the major facilitator superfamily.
TLDR
A structural genomics approach for the amplified expression, purification and characterisation of prokaryotic drug efflux proteins of the 'Major Facilitator Superfamily' of transport proteins from Helicobacter pylori, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Bacillus subtilis, Brucella melitensis, Campylobacter jejuni and Neisseria meningitides is described. Expand
The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands.
TLDR
The pattern of inhibition of NupC by differing nucleoside analogues confirmed the functional relationship of the bacterial transporter to members of the human concentrative nucleosid transporter (CNT) family and reaffirmed the use of theacterial protein as an experimental model for these physiologically and clinically important mammalian proteins. Expand
Antibiotic resistance: multidrug efflux proteins, a common transport mechanism?
TLDR
The focus of the review is the resistance, by efflux, to several structurally unrelated antibiotics by multidrug transport proteins in micro-organisms and higher animals including humans. Expand
Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family
TLDR
Using high-level expression in E. coli and magic-angle spinning cross-polarization solid-state nuclear magnetic resonance, the binding of the permeant to the protein is measured and its relative mobility within the binding site is assessed, under non-energized conditions, opening the way to elucidating the molecular mechanism of transport in this key family of membrane transporters. Expand
Substrate affinities for membrane transport proteins determined by 13C cross-polarization magic-angle spinning nuclear magnetic resonance spectroscopy.
TLDR
Methods in which cross-polarization magic-angle spinning (CP-MAS) solid-state NMR is exploited to measure rigorous parameters for binding of (13)C-labeled substrates to membrane transport proteins are devised, broadly applicable to quantifying binding of substrates, inhibitors, drugs, and antibiotics to numerous membrane proteins. Expand
A new phenazine synthesis. The synthesis of griseoluteic acid, griseolutein A, and methyl diacetylgriseolutein B
2-Nitrodiphenylamines are reductively cyclized to phenazines by sodium borohydride in ethanolic sodium ethoxide solution: the method has been used to provide the first synthesis of griseoluteic acidExpand
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