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Complete genome sequence of the Q-fever pathogen Coxiella burnetii
- R. Seshadri, I. Paulsen, J. Heidelberg
- BiologyProceedings of the National Academy of Sciences…
- 18 April 2003
Analysis of the genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method, suggesting that the obligate intracellular lifestyle of C. burningetii may be a relatively recent innovation.
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis
- R. Heinzen, M. Scidmore, D. Rockey, T. Hackstadt
- Biology, ChemistryInfection and immunity
- 1 March 1996
The data indicate that C. trachomatis replicates within a nonacidified vacuole that is disconnected from endosome-lysosome trafficking but may receive lipid from exocytic vesicles derived from the trans-Golgi network.
Temporal Analysis of Coxiella burnetii Morphological Differentiation
- Sherry A. Coleman, E. Fischer, D. Howe, D. Mead, R. Heinzen
- BiologyJournal of bacteriology
- 1 November 2004
The overall growth cycle of C. burnetii is characteristic of a closed bacterial system and that the replicative form of the organism is the LCV, as indicated by time course transmission electron microscopic analysis and quantitative and qualitative results.
Chlamydia trachomatis interrupts an exocytic pathway to acquire endogenously synthesized sphingomyelin in transit from the Golgi apparatus to the plasma membrane.
The mechanisms of this unusual trafficking and the association of the chlamydial inclusion with the Golgi apparatus are explored and consistent with a model in which C.trachomatis inhabits a unique vesicle which interrupts an exocytic pathway to intercept host sphingolipids in transit from the Gol Gi apparatus to the plasma membrane.
Isolation from Animal Tissue and Genetic Transformation of Coxiella burnetii Are Facilitated by an Improved Axenic Growth Medium
Modified ACCM and culture conditions that support improved growth of C. burnetii genetic tools and provide a sensitive means of primary isolation of the pathogen from Q fever patients are described.
Host cell-free growth of the Q fever bacterium Coxiella burnetii
- Anders Omsland, D. Cockrell, R. Heinzen
- BiologyProceedings of the National Academy of Sciences
- 17 March 2009
Axenic cultivation of C. burnetii will facilitate studies of the organism's pathogenesis and genetics and aid development of Q fever preventatives such as an effective subunit vaccine and may be broadly applicable to development of axenic media that support growth of other medically important obligate intracellular pathogens.
Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii
Current understanding of the cellular events that occur during parasitism of host cells by Coxiella, including deployment of a type IV secretion system to deliver effector proteins to the host cytosol is summarized.
The Chlamydia trachomatis Plasmid Is a Transcriptional Regulator of Chromosomal Genes and a Virulence Factor
A comprehensive molecular and biological characterization of the naturally occurring plasmidless human C. trachomatis strain L2(25667R) is described, suggesting that virulence is controlled, at least in part, by theplasmid's ability to regulate the expression of chromosomal genes.
Comparative Genomics Reveal Extensive Transposon-Mediated Genomic Plasticity and Diversity among Potential Effector Proteins within the Genus Coxiella
The observation that the attenuated Dugway isolate has the largest genome with the fewest pseudogenes and IS elements suggests that this isolate's lineage is at an earlier stage of pathoadaptation than the NM, K, and G lineages.