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The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup.
TLDR
An expert workgroup formed by the International Workshop on Genotoxicity Testing considered the state of assay development and the potential of the assay for regulatory use, and consensus was reached on what is known about the Pig-a assay and how it should be conducted. Expand
Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N-ethyl-N-nitrosourea.
TLDR
The results indicate that ENU-induced Pig-A mutant RBCs accumulate in a near additive fashion in rats, and once present in the peripheral blood, persist for at least 6 months, and could be important for detecting weak mutagens by repeated or subchronic/chronic dosing protocols. Expand
Reduction of nitroaromatic compounds by anaerobic bacteria isolated from the human gastrointestinal tract.
TLDR
Human intestinal microbial flora were screened for their abilities to reduce nitroaromatic compounds by growing them on brain heart infusion agar plates containing 1-nitropyrene and an activity stain for the detection of nitroreductase on anaerobic native polyacrylamide gels showed that the nit mirroreductases from different bacteria had distinct electrophoretic mobilities. Expand
The in vivo pig‐a gene mutation assay, a potential tool for regulatory safety assessment
TLDR
Based on data from a limited number of test agents, the Pig‐a mutation assay shows promise for regulatory applications, including integration of gene mutation measurement into repeat‐dose toxicology studies. Expand
Genotypic selection methods for the direct analysis of point mutations.
TLDR
It is concluded that proficient genotypic selection requires more than one allele-enrichment technique with at least one of these preceding a high-fidelity PCR amplification step. Expand
Nitro group orientation, reduction potential, and direct‐acting mutagenicity of nitro‐polycyclic aromatic hydrocarbons
TLDR
A general finding is that nitro‐PAHs with their nitro substituent oriented perpendicular to the aromatic system exhibit either very weak or no direct‐acting mutagenicity in S. typhimurium strains TA98 and TA100. Expand
Genotoxicity of silver nanoparticles evaluated using the Ames test and in vitro micronucleus assay.
TLDR
The results demonstrate that the 5 nm AgNP are genotoxic in TK6 cells and suggest that the in vitro micronucleus assay may be more appropriate than the Ames test for evaluating the genotoxicity of the AgNPs. Expand
Transgenic animal models in toxicology: historical perspectives and future outlook.
TLDR
This review provides an overview on the applications of transgenic animal models in the assessment of mutagenicity and carcinogenicity, their use as reporter systems, and as tools for understanding the roles of xenobiotic-metabolizing enzymes and biological receptors in the etiology of chemical toxicity. Expand
Comparative analysis of micronuclei and DNA damage induced by Ochratoxin A in two mammalian cell lines.
TLDR
Micronucleus induction by OTA is evaluated in comparison with its ability to induce cytotoxicity and DNA damage in two mammalian cell lines, CHO-K1-BH(4) Chinese hamster ovary cells and TK6 human lymphoblastoid cells to suggest its involvement in producing OTA-induced clastogenicity and aneugenicity is investigated. Expand
In vivo genotoxicity of furan in F344 rats at cancer bioassay doses.
TLDR
The data suggest that furan induces cancer in rat livers mainly through a secondary genotoxic mechanism involving oxidative stress, accompanied by inflammation, cell proliferation, and toxicity. Expand
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