A Herpesvirus Associated with Mass Mortality of Juvenile and Adult Koi, a Strain of Common Carp.
It is strongly suspect that this newly recognized koi herpesvirus (KHV) has the potential to be a significant cause of mortality among koi and presumably common carp.
Recent Advances in Our Knowledge of the Myxozoa
It is indicated that the Myxozoa are closely related to Cnidaria, and marine taxa at the genus level branch separately from genera that usually infect freshwater fishes; taxa cluster more by development and tissue location than by spore morphology.
Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR.
- O. Gilad, S. Yun, Francisco J. Zagmutt-Vergara, C. Leutenegger, H. Bercovier, R. Hedrick
- Biology, Environmental ScienceDiseases of Aquatic Organisms
- 8 September 2004
Although there were no recognized patterns to viral DNA concentrations as found in different tissues over time, KHV genome copies for all tissues increased with time post virus exposure and with water temperature.
Genome Sequences of Three Koi Herpesvirus Isolates Representing the Expanding Distribution of an Emerging Disease Threatening Koi and Common Carp Worldwide
The conclusion that at least some of these mutations occurred in vivo prompts the hypothesis that loss of gene functions might be associated with emergence of the disease and provides a basis for further investigations into the molecular epidemiology of the virus.
Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi.
Initial characteristics of the virus show that it is clearly different from Herpesvirus cyprini (CHV), the most commonly known herpesvirus from cyprinid fish, and the genomic DNA restriction fragments clearly distinguish KHV from CHV and CCV.
Molecular characterization, sequence analysis, and taxonomic position of newly isolated fish iridoviruses.
It is demonstrated that the piscine iridoviruses examined here were members of the genus Ranavirus, and surveys of pathogenic "fish viruses" may need to include neighboring amphibian and reptilian populations, and PCR will be useful in the diagnosis of fish disease.
Paramoeba pemaquidensis (Sarcomastigophora: Paramoebidae) infestation of the gills of coho salmon Oncorhynchus kisutch reared in sea water.
In vitro studies indicated that the optimal salinity for growth of the amoeba was between 15 and 20 ppt, while growth was greatly dminished below 10 pPT, which suggests that the organism is an opportunistic pathogen which only proliferates on fish gills and causes disease under certain situations yet to be clearly defined.
Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis
The TK based PCR assay developed in this work was shown to be specific for the detection of KHV and was as sensitive as virus isolation which is the golden standard method for KHV diagnosis and was able to detect as little as 10 fentograms of KHVs DNA corresponding to 30 virions.
Proliferative kidney disease of salmonid fish
Relationships among members of the genus Myxobolus (Myxozoa: Bilvalvidae) based on small subunit ribosomal DNA sequences.
- K. Andree, C. Székely, K. Molnár, S. J. Gresoviac, R. Hedrick
- BiologyJournal of Parasitology
- 1 February 1999
It is proposed that 18S rRNA gene sequences, combined with information on tissue tropism, host species infected, and developmental cycles in the fish and alternate host (when and if known) be given greater consideration in taxonomic placements of myxosporeans.