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Crystal and Molecular Structure of Barley α-Amylase
The three-dimensional structure of barley malt α-amylase (isoform AMY2-2) was determined by multiple isomorphous replacement using three heavy-atom derivatives and solvent flattening. The model wasExpand
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The structure of barley alpha-amylase isozyme 1 reveals a novel role of domain C in substrate recognition and binding: a pair of sugar tongs.
Though the three-dimensional structures of barley alpha-amylase isozymes AMY1 and AMY2 are very similar, they differ remarkably from each other in their affinity for Ca(2+) and when interacting withExpand
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The crystal structure of HasA, a hemophore secreted by Serratia marcescens
Free iron availability is strongly limited in vertebrate hosts, making the iron acquisition by siderophores inappropriate. Pathogenic bacteria have developed various ways to use the host's iron fromExpand
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Three dimensional structure of porcine pancreatic alpha‐amylase at 2.9 A resolution. Role of calcium in structure and activity.
The crystal structure of porcine pancreatic alpha‐amylase (PPA) has been solved at 2.9 A resolution by X‐ray crystallographic methods. The enzyme contains three domains. The larger, in the N‐terminalExpand
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Trehalulose Synthase Native and Carbohydrate Complexed Structures Provide Insights into Sucrose Isomerization*
Various diseases related to the overconsumption of sugar make a growing need for sugar substitutes. Because sucrose is an inexpensive and readily available d-glucose donor, the industrial potentialExpand
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The crystal structure of the processive endocellulase CelF of Clostridium cellulolyticum in complex with a thiooligosaccharide inhibitor at 2.0 Å resolution
The mesophilic bacterium Clostridium cellulolyticum exports multienzyme complexes called cellulosomes to digest cellulose. One of the three major components of the cellulosome is the processiveExpand
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Structures of mutants of cellulase Cel48F of Clostridium cellulolyticum in complex with long hemithiocellooligosaccharides give rise to a new view of the substrate pathway during processive action.
An efficient breakdown of lignocellulosic biomass is a prerequisite for the production of second-generation biofuels. Cellulases are key enzymes in this process. We crystallized complexes betweenExpand
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X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides.
Complete cellulose degradation is the first step in the use of biomass as a source of renewable energy. To this end, the engineering of novel cellulase activity, the activity responsible for theExpand
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Sequence, structure and activity of phosphoglycerate kinase: a possible hinge-bending enzyme
The fitting of sequenced peptides to a high-resolution X-ray map of phosphoglycerate kinase has yielded the complete sequence and structure of the horse muscle enzyme. Metal ADP and ATP substratesExpand
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Structure and Molecular Model Refinement of Pig Pancreatic α-Amylase at 2·1 Å Resolution
Abstract The preciously reported structural model of porcine pancreatic α-amylase has been corrected and improved by a genuinely independent structure solution. The electron density map wasExpand
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