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Universal primer cocktails for fish DNA barcoding
The results show that M13-tailed primer cocktails are more effective than conventional degenerate primers, allowing barcode work on taxonomically diverse samples to be carried out in a high-throughput fashion.
Identifying Canadian Freshwater Fishes through DNA Barcodes
The present study evidenced that freshwater fish species can be efficiently identified through the use of DNA barcoding, especially the species complex of small-sized species, and that the present COI library can be used for subsequent applications in ecology and systematics.
The campaign to DNA barcode all fishes, FISH-BOL.
Analysis is targeting a 648 base pair region of the mitochondrial cytochrome c oxidase I (COI) gene, targeting several specimens with divergent barcode sequences confirmed by integrative taxonomic analysis as new species.
Genetic calibration of species diversity among North America's freshwater fishes
- J. April, R. Mayden, R. Hanner, L. Bernatchez
- Environmental Science, BiologyProceedings of the National Academy of Sciences
- 13 June 2011
It appears that the current North American freshwater fish taxonomy at the species level significantly conceals diversity in some groups, although artificially creating diversity in others, according to a survey of named species.
Can DNA barcoding accurately discriminate megadiverse Neotropical freshwater fish fauna?
This study is the first to examine a large number of freshwater fish species from the Neotropical area, including alarge number of closely related species, and confirmed the efficacy of the barcoding methodology to identify a recently radiated, megadiverse fauna.
Phylogeny of the Acipenseriformes: cytogenetic and molecular approaches
The phylogeny based on molecular analysis had two major departures from existing morphological hypotheses: Huso dauricus is a sister-species to Acipenser instead of being basal to all acipenseriforms, and Scaphirhynchus and Pseudoscaphirchus do not form a monophyletic group.
DNA barcoding detects market substitution in North American seafood
Cryptic species in a "living fossil" lineage: taxonomic and phylogenetic relationships within the genus Lepidurus (Crustacea: Notostraca) in North America.
The results point to the existence of cryptic species within the current classification scheme for Lepidurus, the need for further taxonomic work within the Notostraca in general, and the role that genetic techniques can play in clarifying the systematics of morphologically conservative groups.
DNA barcoding identifies Eimeria species and contributes to the phylogenetics of coccidian parasites (Eimeriorina, Apicomplexa, Alveolata).
A single-laboratory validated method for the generation of DNA barcodes for the identification of fish for regulatory compliance.
A protocol with single-laboratory validation for the generation of DNA barcodes suitable for the identification of seafood products, specifically fish, in a manner that is suitable for FDA regulatory use is provided.