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A quick and inexpensive method for removing polysaccharides from plant genomic DNA.
The results show that high-salt precipitation effectively removed polysaccharides and their inhibitory effects on restriction enzyme and Taq polymerase activity. Expand
QTLseqr: An R Package for Bulk Segregant Analysis with Next‐Generation Sequencing
An R package that performs Next Generation Sequencing Bulk Segregant Analysis was developed and is quick and produces publication quality figures and tables. Expand
Interaction between zucchini yellow mosaic potyvirus RNA-dependent RNA polymerase and host poly-(A) binding protein.
This is the first report of an interaction between a viral replicase and P ABP and may implicate a role for host PABP in the potyviral infection process. Expand
Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants
Transgenic muskmelon (Cucumis melo L.) plants were produced efficiently by inoculating cotyledon explants with Agrobacterium tumefaciens strain LBA4404 bearing a Ti plasmid with the NPT II gene forExpand
CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection[OPEN]
Evidence is presented that CAMTA3-mediated repression of SA pathway genes in nonstressed plants involves the action of an N-terminal repression module (NRM) that acts independently of calmodulin (CaM) binding to the IQ and CaM binding (CaMB) domains, a finding that is contrary to current thinking that CAMta3 repression activity requires binding of CaM to the CaMB domain. Expand
Resistance of Cucumber Cultivars to a New Strain of Cucurbit Downy Mildew
The best cultivars in this study were only moderately resistant and would likely require fungicide applications to achieve high yield and quality in the presence of downy mildew, although differences in resistance were detected. Expand
Transcriptome analyses of early cucumber fruit growth identifies distinct gene modules associated with phases of development
The results suggest that the interval between expansive growth and ripening includes further developmental differentiation with an emphasis on defense and the increased transcript levels of cucurbit-specific genes during the exponential growth stage may indicate unique factors contributing to rapid growth in cucurbits. Expand
A Detached Cucumber Fruit Method to Screen for Resistance to Phytophthora capsici and Effect of Fruit Age on Susceptibility to Infection.
This is the first report using a nonwounded fruit screen to analyze cucumber resistance to P. capsici, and it was observed that younger, smaller fruit were comparatively more susceptible than older, larger fruit. Expand
Melon fruits: genetic diversity, physiology, and biotechnology features.
The present paper describes the various research findings related to successful approaches to plant regeneration and transgenic transformation of C. melo and describes potential improvement of melon to improve fruit quality characteristics and postharvest handling. Expand
Manipulation of plant architecture to enhance crop disease control
These examples demonstrate that breeding for modified plant architecture can be a valuable component of a broader disease control strategy that also includes genetic resistance and cultural and chemical controls. Expand