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Refined crystal structure of the triphosphate conformation of H‐ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.
- E. Pai, U. Krengel, G. Petsko, R. Goody, W. Kabsch, A. Wittinghofer
- ChemistryThe EMBO journal
- 1 August 1990
A mechanism for GTP hydrolysis involving mainly Gln61 and Glu63 as activating species for in‐line attack of water as well as a mechanism for rate enhancement by GAP is proposed.
The Legionella Effector Protein DrrA AMPylates the Membrane Traffic Regulator Rab1b
The ability of Legionella pneumophila to manipulate vesicular trafficking by the covalent modification of the small guanosine triphosphatase (GTPase) Rab1, which normally regulates the transport of endoplasmic reticulum–derived vesicles in eukaryotic cells is described.
The original Michaelis constant: translation of the 1913 Michaelis-Menten paper.
This reanalysis of Michaelis and Menten's data using modern computational methods revealed an unanticipated rigor and precision in the original publication and uncovered a sophisticated, comprehensive analysis that has been overlooked in the century since their work was published.
Kinetics of interaction of nucleotides with nucleotide-free H-ras p21.
- J. John, R. Sohmen, J. Feuerstein, R. Linke, A. Wittinghofer, R. Goody
- Biology, ChemistryBiochemistry
- 26 June 1990
By use of a nonequilibrium competition method, guanosine and GMP have been shown to interact weakly but significantly with p21, leading to a marked stabilization of p21 against spontaneous denaturation when compared with the nucleotide- and nucleoside-free protein.
Structure of the N6-adenine DNA methyltransferase M•TaqI in complex with DNA and a cofactor analog
- K. Goedecke, M. Pignot, R. Goody, A. Scheidig, E. Weinhold
- Chemistry, BiologyNature Structural Biology
- 1 February 2001
The 2.0 Å crystal structure of the N6-adenine DNA methyltransferase M•TaqI in complex with specific DNA and a nonreactive cofactor analog reveals a previously unrecognized stabilization of the…
Structure of the Rab7:REP-1 Complex Insights into the Mechanism of Rab Prenylation and Choroideremia Disease
A toolkit and benchmark study for FRET-restrained high-precision structural modeling
A dramatic improvement in the precision of FRET-derived structures is achieved by explicitly considering spatial distributions of dye positions, which greatly reduces uncertainties due to flexible dye linkers.
GrpE accelerates nucleotide exchange of the molecular chaperone DnaK with an associative displacement mechanism.
- L. Packschies, H. Theyssen, A. Buchberger, B. Bukau, R. Goody, J. Reinstein
- Biology, ChemistryBiochemistry
- 25 March 1997
The authors' data indicate that the initial weak binding of GrpE to DnaK x ADP is followed by an isomerization of the ternary complex which leads to weakening of nucleotide binding and finally to its rapid dissociation.
The kinetic mechanism of Ran--nucleotide exchange catalyzed by RCC1.
The quantitative results of the kinetic analysis suggest that the exchange reaction does not per se favor the formation of the Ran.GTP complex, but rather accelerates the formationof the equilibrium dictated by the relative affinities of Ran for GDP/GTP and the respective concentrations of the nucleotide in the cell.
RabGEFs are a major determinant for specific Rab membrane targeting
Analysis of three different Rab-RabGEF pairs reveals that RabGEFs contain the minimal targeting machinery for recruiting Rabs to specific membranes.