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Oral Biofilm Architecture on Natural Teeth
- V. Zijnge, M. V. van Leeuwen, +4 authors Hermie J. M. Harmsen
- Medicine, BiologyPloS one
- 24 February 2010
In vivo observations on oral biofilms provide a clear vision on biofilm architecture and the spatial distribution of predominant species and identified for the first time in vivo that Lactobacillus sp.
Multiplex FISH analysis of a six-species bacterial biofilm.
Results show that the simultaneous investigation by FISH of complex biofilms composed of multiple bacterial species with differential Gram-staining properties is possible and holds great promise for the analysis of spatial alterations in biofilm composition in response to environmental challenges.
Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies
One monoclonal antibody was directed against an antigen strongly expressed on all saccharolytic black-pigmenting Bacteroides strains tested so far, thus confirming the previously noted antigenic relationship between the species which had emerged from the former B. intermedius and B. melaninogenicus groups.
Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque
- T. Thurnheer, R. Gmür, S. Shapiro, B. Guggenheim
- Medicine, ChemistryApplied and Environmental Microbiology
- 1 March 2003
Analysis of diffusion phenomena through the biofilms suggested tortuosity as the most probable explanation for retarded diffusion, as selective attenuated diffusion of fermentable carbohydrates and acids produced within dental plaque is thought to be essential for the development of carious lesions.
Actinobacillus actinomycetemcomitans in Chinese adults. Serotype distribution and analysis of the leukotoxin gene promoter locus.
- A. Mombelli, R. Gmür, N. Lang, E. Corbert, J. Frey
- Biology, MedicineJournal of clinical periodontology
- 1 August 1999
A high prevalence irrespective of gender, age, and cohort suggests that A. actinomycetemcomitans is a common constituent of the normal flora in the Chinese subjects of this study and suggests differences in the microbiological composition of subgingival plaque may exist for this population group as compared to north American and European populations.
In vitro modeling of host-parasite interactions: the 'subgingival' biofilm challenge of primary human epithelial cells
An experimental in vitro model to study processes taking place in the gingival crevice during the initiation of inflammation is developed, taking into account that the microbial challenge derives from a biofilm community and not from planktonically cultured bacterial strains.
In vitro quantitative light-induced fluorescence to measure changes in enamel mineralization
- R. Gmür, E. Giertsen, M. H. Veen, E. Josselin de Jong, J. M. Cate, B. Guggenheim
- Chemistry, MedicineClinical Oral Investigations
- 30 June 2006
The ability of in vitro quantitative light-induced fluorescence (QLF) to quantify changes in mineralization of bovine enamel discs that had been exposed in vitro to a demineralizing gel or biofilm-mediated dem ineralization challenges is demonstrated.
Application of the Zürich Biofilm Model to Problems of Cariology
- B. Guggenheim, M. Guggenheim, R. Gmür, E. Giertsen, T. Thurnheer
- Medicine, BiologyCaries Research
- 21 May 2004
A biofilm model developed in Zürich is described and a number of applications with direct or indirect impact on prophylactic dentistry are demonstrated: spatial arrangement and associative behavior of various species in biofilms; multiplex fluorescent in situ hybridization analysis of oral bacteria in biofilmms; use of the bio film model to predict in vivo efficacy of antimicrobials reliably.
Uncultivated Tannerella BU045 and BU063 are slim segmented filamentous rods of high prevalence but low abundance in inflammatory disease-associated dental plaques.
Data demonstrate that Tannerella phylotypes BU045, BU063, 127+ and 997+ consist of long slim rods with segments, which, with respect to FISH stainability, often behaved as independent units, which seems to exclude any relevance for the pathogenesis of periodontal diseases.
Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms
Application of these new group- and species-specific FISH probes to oral biofilm-forming lactic acid bacteria will allow a clearer understanding of the supragingival biome, its spatial architecture and of structure-function relationships implicated during plaque homeostasis and caries development.