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more acidic solutions, with pH < 7 0, a few seconds elapsed before equilibrium was attained. Taking into account the ionization of the haem-linked group on MetMb and the higher oxidation state, the… (More)
A LARGE number of enzymes have been located in the mitochondria fraction isolated by means of differential centrifugation; this fact is generally assumed to indicate that they are structurally… (More)
A partially purified extract was obtained of a rat-liver fraction containing the mitochondria and lysosomes. Two components with β-glucuronidase activity were separated from it. Both showed the same… (More)
Abstract β-Glucuronidase (β- d -glucuronide glucuronohydrolase, EC 18.104.22.168) from rat-liver lysosomes was purified by (NH4)2SO4 precipitation, gel filtration on Sephadex G-200, treatment with… (More)
One of the sulphatases of rat liver, like acid phosphatase, is enclosed within cytoplasmic granules. The two enzymes are released from these granules in similar proportions by various treatments.
Summary Crude, soluble β-glucuronidase (EC 22.214.171.124) preparations from rat-liver lysosomes and microsomes were submitted to disc electrophoresis on polyacrylamide gel. Under the experimental… (More)