• Publications
  • Influence
Crystal structure analysis of NP24-I: a thaumatin-like protein
TLDR
NP24 has been reported to be anallergenic protein and an allergenic motif could be identified on the surface of the helical domain II of NP24-I, a feature common to other antifungal TLPs. Expand
Structural and mutational analysis of archaeal ATP-dependent RNA ligase identifies amino acids required for RNA binding and catalysis
TLDR
X-ray crystal structures of an ATP-dependent RNA ligase from Methanobacterium thermoautotrophicum as well as the covalent MthRnl–AMP intermediate are reported and an RNA binding interface is proposed based on the mutational studies and two sulfate ions that co-crystallized at the active site cleft in the MstRnl-AMP complex. Expand
Tumor suppressor p53-mediated structural reorganization of the transcriptional coactivator p300.
TLDR
The 3D cryo-EM density map of the p300-p53 complex revealed that substantial change in the relative arrangement of Bromo and HAT domains occurs upon complex formation which is likely required for exposing HAT active site and subsequent acetyltransferases activity. Expand
Structural insights into the substrate specificity and activity of ervatamins, the papain‐like cysteine proteases from a tropical plant, Ervatamia coronaria
TLDR
The studies indicate a higher enzymatic activity of ervatamin‐A, which has been subsequently explained at the molecular level from the three‐dimensional structure of the enzyme and in the context of its helix polarizibility and active site plasticity. Expand
A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies.
TLDR
The cloning and characterization of the entire cDNA of a papain-like cysteine protease from a tropical flowering plant shows that the extended peptide stretch region of the N-terminal propeptide, covering the interdomain cleft, contains protruding side chains of positively charged residues. Expand
Heterologous expression of a thermostable plant cysteine protease in Escherichia coli both in soluble and insoluble forms
TLDR
The recombinant enzyme shows a similar enzymatic activity, specificity and thermal stability pattern like its native counterpart when expressed in Escherichia coli using two T7 based expression vectors. Expand
Ultrafast differential flexibility of Cro-protein binding domains of two operator DNAs with different sequences.
TLDR
This study mutated (site directed mutagenesis: SDM) Cro-protein at the 37th position with a cysteine residue retaining the functional integrity of the protein and labelled the cysteINE residue, which is close to the interface, with a fluorescent probe (AEDANS), for the investigation of its interface with operator DNAs (OR3 and OR2). Expand
Crystallization and preliminary X-ray diffraction studies of NP24-I, an isoform of a thaumatin-like protein from ripe tomato fruits.
TLDR
NP24 is a 24 kDa (207-amino-acid) antifungal thaumatin-like protein (TLP) found in tomato fruits that has been isolated and purified and crystallized by the hanging-drop vapour-diffusion method. Expand
Enhanced basepair dynamics pre-disposes protein-assisted flips of key bases in DNA strand separation during transcription initiation.
TLDR
Results suggest that flipping of -11A, and perhaps other flipped bases observed in the open-complex, is facilitated by its inherent proclivity to open-up with further assistance from the protein, thus leading to a strand-open state. Expand
The Wild-type and Gain-of-function Mutant p53 Enhance p300 Autoacetylation through Conformational Switching
TLDR
It is concluded that allosteric activation of p300 by p53 underpins gene regulation by p 53, and reversal of gain-of-function properties of mutant p53 suggests that molecules targeting the p53-p300 interface may be good candidates for anti-tumor drugs. Expand
...
1
2
...