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A Conserved Mechanism for Centromeric Nucleosome Recognition by Centromere Protein CENP-C
Kinetochore Targeting Chromosomes must be segregated accurately during cell division. This is facilitated by the attachment of mitotic spindle microtubules to the kinetochore at the chromosomalExpand
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Structure of the MutL C‐terminal domain: a model of intact MutL and its roles in mismatch repair
MutL assists the mismatch recognition protein MutS to initiate and coordinate mismatch repair in species ranging from bacteria to humans. The MutL N‐terminal ATPase domain is highly conserved, butExpand
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Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription.
We report here crystal structures of human RNase H1 complexed with an RNA/DNA substrate. Unlike B. halodurans RNase H1, human RNase H1 has a basic protrusion, which forms a DNA-binding channel andExpand
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Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex.
Barrier-to-autointegration factor (BAF) is a highly conserved cellular protein that was identified by its activity in protecting retroviral DNA against autointegration. We show that BAF has theExpand
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Crystal Structure of the 14-3-3ζ:Serotonin N-Acetyltransferase Complex A Role for Scaffolding in Enzyme Regulation
Serotonin N-acetyltransferase (AANAT) controls the daily rhythm in melatonin synthesis. When isolated from tissue, AANAT copurifies with isoforms epsilon and zeta of 14-3-3. We have determined theExpand
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Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3
The centromere is a unique chromosomal locus that ensures accurate segregation of chromosomes during cell division by directing the assembly of a multiprotein complex, the kinetochore. The centromereExpand
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The influence of glycosylation on the thermal stability and effector function expression of human IgG1-Fc: properties of a series of truncated glycoforms.
Antibodies are multifunctional molecules that following the formation of antibody antigen complexes, may activate mechanisms to effect the clearance and destruction of the antigen (pathogen). The IgGExpand
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Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM‐domain structures: one binds BAF and the other binds DNA
The nuclear envelope proteins LAP2, emerin and MAN1 share a conserved ∼40‐residue ‘LEM’ motif. Loss of emerin causes Emery–Dreifuss muscular dystrophy. We have solved the solution NMR structure ofExpand
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Midbody Targeting of the ESCRT Machinery by a Noncanonical Coiled Coil in CEP55
The ESCRT (endosomal sorting complex required for transport) machinery is required for the scission of membrane necks in processes including the budding of HIV-1 and cytokinesis. An essential step inExpand
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Acyldepsipeptide antibiotics induce the formation of a structured axial channel in ClpP: A model for the ClpX/ClpA-bound state of ClpP.
In ClpXP and ClpAP complexes, ClpA and ClpX use the energy of ATP hydrolysis to unfold proteins and translocate them into the self-compartmentalized ClpP protease. ClpP requires the ATPases toExpand
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