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Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro.
Analysis of spectral and chemical data indicates that the major product of the interaction of metabolically activated aflatoxin B1 and DNA is 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B 1 with the guanine and hydroxyl functions possessing a trans configuration. Expand
Identification of the principal aflatoxin B1-DNA adduct formed in vivo in rat liver.
Quantitative studies of formation of the principal covalent product formed in liver DNA of rats treated with AFB1 proved that the major adduct formed between DNA and AFB1 in vivo is identical to that produced in vitro when AFB1 is incubated with DNA in the presence of a rat liver microsomal activating system. Expand
Mutational spectra of aflatoxin B1 in vivo establish biomarkers of exposure for human hepatocellular carcinoma
High-fidelity DNA sequencing and a mouse model are used to reveal high-resolution mutational spectra of the liver carcinogen aflatoxin B1 in histopathologically normal liver as early as 10 wk after exposure, proposing that the 10-wk HRMS reflects a short-term mutational response to AFB1, and is an early detection metric for AFB1-induced liver cancer in this mouse model. Expand
Benzo[a]pyrene Metabolites: Efficient and Rapid Separation by High-Pressure Liquid Chromatography
High-pressure liquid chromatography can separate eight metabolites of benzo[a] pyrene formed by rat liver microsomes. This method offers major advantages over previous techniques used for theExpand
Temporal patterns of covalent DNA adducts in rat liver after single and multiple doses of aflatoxin B1.
Administration of multiple doses of aflatoxin B1, using a regimen shown to produce a high incidence of hepatocellular carcinoma, caused accumulation of these persistent products in liver DNA over a 14-day period. Expand
Quantitative comparison of covalent aflatoxin-DNA adducts formed in rat and mouse livers and kidneys.
In vitro studies on the activation of AFB1 by microsomal fractions of mouse and rat livers found that mouse liver microsomes were rapidly inactivated, and a good correlation was found between the level of covalent modification of DNA, species sensitivity, and organ specificity, to the toxic effects of AFB 1. Expand
Disruption of gene expression and induction of apoptosis in prostate cancer cells by a DNA-damaging agent tethered to an androgen receptor ligand.
11beta-dichloro induces a unique pattern of gene disruption, induces apoptosis in apoptosis-resistant cells, and shows promising anticancer activity in animals. Expand
Metabolism of benzo(a)pyrene by human epithelial cells in vitro.
The findings suggest that the prevalence of carcinomatous disease in humans is due to the differential capacity of the epithelial cells to metabolize potential carcinogens to active forms, a capacity reduced in fibroblasts or other nonepithelial cells. Expand
Enhanced synthesis and stabilization of Mr 68,000 protein in transformed BALB/c-3T3 cells: candidate for restriction point control of cell growth.
  • R. G. Croy, A. Pardee
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 August 1983
This protein could be important in determining the loss of growth regulation in these tumor cells and is searched for using two-dimensional electrophoresis to resolve protein from cells labeled with [35S]methionine to find one that has a greater rate of synthesis and stability in benzo[a]pyrene-transformed than in untransformed cells. Expand