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New Method for Typing Staphylococcus aureus Strains: Multiple-Locus Variable-Number Tandem Repeat Analysis of Polymorphism and Genetic Relationships of Clinical Isolates
TLDR
The PCR-based methodology applied to multiple-locus variable numbers of tandem repeat (VNTR) analysis was recently shown to be a useful technique for the molecular typing of clinical isolates of several bacterial species and it was shown that the method allows typing of S. aureus strains with the discriminatory power and reproducibility of pulsed-field gel electrophoresis. Expand
Staphostatins resemble lipocalins, not cystatins in fold
TLDR
The 1.4 Å crystal structure of staphostatin B is presented and it is shown that the fold can be described as a fully closed, highly sheared eight‐stranded β‐barrel. Expand
Crystal Structure of a Fragment of Mouse Ubiquitin-activating Enzyme*
TLDR
A biochemical and crystallographic characterization of the two half-domains and the crystal structure of the larger, second catalytic cysteine half-domain of mouse ubiquitin-activating enzyme are presented and a tentative model for full-length ubiquitIn-Activating enzyme is proposed. Expand
The Staphostatin-Staphopain Complex
TLDR
Unexpectedly for a cysteine protease inhibitor, staphostatin B binds to staphopain B in an almost substrate-like manner and the inhibitor polypeptide chain runs through the protease active site cleft in the forward direction. Expand
Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition.
TLDR
In a modeled complex of prostaphopain B with staphostatin B, clashes occur both inside and outside the active site cleft, but involve mostly poorly ordered regions of the protein that may be mobile. Expand
A Comparison of Staphostatin B with Standard Mechanism Serine Protease Inhibitors*
TLDR
The crystal structure of staphostatin B in complex with wild-type staphopain B at 1.9 Å resolution is reported, strikingly similar to the “ionmolecule” arrangement for the complex of papain-type enzymes with their substrates but differs significantly from the arrangement conventionally assumed. Expand
Genetic characterization of staphopain genes in Staphylococcus aureus
TLDR
Taken together, data suggest that the staphopains have important housekeeping and/or virulence functions, and therefore may constitute an interesting target for the development of therapeutic inhibitors for the treatment of staphylococcal diseases. Expand
Fighting an enemy within: cytoplasmic inhibitors of bacterial cysteine proteases
TLDR
Bioinformatic analysis of other prokaryotic genomes has revealed that two more species may utilize this same genetic arrangement to control streptopain‐like proteases with lipocalin‐like inhibitors, while three other species may employ a C‐terminally located domain that resembles the profragment. Expand
Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants of Nicotianatabacum
TLDR
Diffraction of Photosystem II and CP43 crystals at various synchrotron beamlines was limited to a resolution of 7 and 14 Å, respectively, and the diffraction quality was insufficient for an unambiguous assignment of the crystallographic lattice or space group. Expand
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