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NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1
Classic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP−CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs.Expand
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.
The crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA is determined, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base in a pocket of the protein. Expand
Crystal Structure of the Nuclear Export Receptor CRM1 in Complex with Snurportin1 and RanGTP
The structure of an exportin complex shows how nuclear transport complexes differentially recognize cargo and suggests that RanGTP promotes cargo-binding to CRM1 solely through long-range conformational changes in the exportin. Expand
Structure of the E. coli ribosome–EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM
The refined cryo-EM-based model presents the currently most complete high-resolution structure of the E. coli ribosome, which demonstrates the power of cryo -EM in structure determination of large and dynamic macromolecular complexes. Expand
Crystal structure of the surfactin synthetase‐activating enzyme Sfp: a prototype of the 4′‐phosphopantetheinyl transferase superfamily
The structure of the Sfp–CoA complex determined at 1.8 Å resolution reveals a novel α/β‐fold exhibiting an unexpected intramolecular 2‐fold pseudosymmetry, which suggests a similar fold and dimerization mode for the homodimeric phosphopantetheinyl transferases such as acyl carrier protein synthase. Expand
Functional interaction of a novel 15.5kD[U4/U6·U5] tri‐snRNP protein with the5′ stem–loop of U4 snRNA
The finding that the 15.5kD protein also efficiently binds to the 5′ stem–loop of U4 atac snRNA indicates that it may be shared by the [U4atac/U6atac·U5] tri‐snRNP of the minor U12‐type spliceosome. Expand
Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus.
The well ordered cytoplasmic C terminus of the protein in the AmT-1 structure has allowed for the construction of a docking model between Amt-1 and a homology model for its physiological interaction partner, the P(II) protein GlnB-1. Expand
Isolation, crystallization, crystal structure analysis and refinement of allophycocyanin from the cyanobacterium Spirulina platensis at 2.3 A resolution.
The phycobiliprotein allophycocyanin from the cyanobacterium Spirulina platensis has been isolated and crystallized and the molecular structure of the subunits resembles other solved phyCobilip protein structures. Expand
Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components
The data suggest that Prp2 facilitates catalytic activation by remodeling the spliceosome, including destabilizing the SF3a and SF3b proteins, likely exposing the branch site before step 1. Expand
Crystal structure of the polysialic acid–degrading endosialidase of bacteriophage K1F
The crystal structures of endosialidase NF and its complex with oligomeric sialic acid indicate that the unique polySia specificity evolved from a combination of structural elements characteristic of exosilidases and bacteriophage tailspike proteins. Expand