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Improvement of enzyme activity, stability and selectivity via immobilization techniques
Abstract In spite of their excellent catalytic properties, enzyme properties have to be usually improved before their implementation at industrial scale (where many cycles of high yield processes areExpand
Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance
Enzyme biocatalysis plays a very relevant role in the development of many chemical industries, e.g., energy, food or fine chemistry. To achieve this goal, enzyme immobilization is a usualExpand
Lipase from Thermomyces lanuginosus: Uses and prospects as an industrial biocatalyst
The lipase from Thermomyces laguginosus (formerly Humicola laguginosa) (TLL) is a basophilic and noticeably thermostable enzyme, commercially available in both soluble and immobilized form. AlthoughExpand
Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization
Glutaraldehyde is one of the most widely used reagents in the design of biocatalysts. It is a powerful crosslinker, able to react with itself, with the advantages that this may bring forth. In thisExpand
Modifying enzyme activity and selectivity by immobilization.
In this tutorial review, some of the main reasons that may produce an improvement in enzyme activity, specificity or selectivity, either real or apparent, due to immobilization are listed. Expand
Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements*
The combination of structural and biochemical studies indicate that the lid opening is not mediated by temperature but triggered by interaction with lipid substrate, and the first structure of a member of the lipase family I.5 showing an open configuration is reported. Expand
Epoxy Sepabeads: A Novel Epoxy Support for Stabilization of Industrial Enzymes via Very Intense Multipoint Covalent Attachment
Sepabeads‐EP (a new epoxy support) has been utilized to immobilize‐stabilize the enzyme penicillin G acylase via multipoint covalent attachment and was hundreds‐fold more stable than Eupergit C derivatives when using a more sophisticated three‐step immobilization/stabilization/blockage procedure. Expand
Advances in the design of new epoxy supports for enzyme immobilization-stabilization.
The possibilities of a directed immobilization of mutated enzymes (change of an amino acid by cysteine on specific points of the protein surface) on tailor-made disulfide-epoxy supports will be discussed as an almost-ideal procedure to achieve very intense and very efficient rigidification of a desired region of industrial enzymes. Expand
Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation
Abstract The moderate stability of enzymes is one of the main drawbacks that hinder general implementation of these interesting biocatalysts at industrial scale. An especially complex problem is theExpand
Control of protein immobilization: coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance.
The advances in support design and the deeper knowledge of the mechanisms of enzyme-support interactions have permitted exploration of the possibilities of the coupled use of site-directed mutagenesis and immobilization in a new way. Expand