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Pharmacological characterization of heterologously expressed ATP-gated cation channels (P2x purinoceptors).
cDNAs encoding P2x purinoceptors from human bladder smooth muscle and from rat PC-12 cells were expressed in oocytes and human embryonic kidney 293 cells. Agonist potencies of 2-methylthio-ATP =Expand
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Ionic permeability of, and divalent cation effects on, two ATP‐gated cation channels (P2X receptors) expressed in mammalian cells.
1. Complementary DNAs for the ATP‐gated ion channel subunits P2X1 (from human bladder) and P2X2 (from rat phaeochromocytoma (PC12) cells) were used to express the receptors in human embryonic kidneyExpand
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The Role of Positively Charged Amino Acids in ATP Recognition by Human P2X1 Receptors*
P2X receptors for ATP are a family of ligand-gated cation channels. There are 11 conserved positive charges in the extracellular loop of P2X receptors. We have generated point mutants of theseExpand
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ATP Binding at Human P2X1 Receptors
P2X receptors comprise a family of ATP-gated ion channels with the basic amino acids Lys-68, Arg-292, and Lys-309 (P2X1 receptor numbering) contributing to agonist potency. In many ATP-bindingExpand
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2′,3′‐O‐(2,4,6‐ trinitrophenyl) adenosine 5′‐triphosphate (TNP‐ATP)–a nanomolar affinity antagonist at rat mesenteric artery P2X receptor ion channels
1 P2X receptor activation by α,β‐meATP evoked inward currents in acutely dissociated rat mesenteric artery smooth muscle cells and contractions of whole artery rings. 2 The selective P2X1 and P2X3Expand
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Molecular properties of P2X receptors
P2X receptors for adenosine tri-phosphate (ATP) are a distinct family of ligand-gated cation channels with two transmembrane domains, intracellular amino and carboxy termini and a large extracellularExpand
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Molecular properties of ATP-gated P2X receptor ion channels.
P2X receptors for ATP are expressed throughout the body and mediate a multitude of functions, including muscle contraction, neuronal excitability and bone formation. In the mid-1990s seven genesExpand
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The role of positively charged amino acids in ATP recognition by human P2X(1) receptors.
P2X receptors for ATP are a family of ligand-gated cation channels. There are 11 conserved positive charges in the extracellular loop of P2X receptors. We have generated point mutants of theseExpand
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Conserved cysteine residues in the extracellular loop of the human P2X(1) receptor form disulfide bonds and are involved in receptor trafficking to the cell surface.
P2X receptors contain 10 conserved cysteines in the extracellular loop. To investigate whether these residues form disulfide bonds, we created a series of single and double cysteine-alanine mutantsExpand
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A Role of the Fast ATP-gated P2X1 Cation Channel in Thrombosis of Small Arteries In Vivo
The P2X1 receptor is a fast ATP-gated cation channel expressed in blood platelets, where its role has been difficult to assess due to its rapid desensitization and the lack of pharmacological tools.Expand
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