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Mutations in the fission yeast silencing factors clr4+ and rik1+ disrupt the localisation of the chromo domain protein Swi6p and impair centromere function.
Observations suggest that clr4+ and rik1+ must play a role in the assembly of Swi6p into a transcriptionally silent, inaccessible chromatin structure at fission yeast centromeres which is required to facilitate interactions with spindle microtubules and to ensure normal chromosome segregation.
FH3, A Domain Found in Formins, Targets the Fission Yeast Formin Fus1 to the Projection Tip During Conjugation
The FH3 domains of both Fus1 and the S. pombe cytokinesis formin Cdc12 were able to localize GFP to the spindle pole body in half of the late G2 cells in a vegetatively growing population, suggesting that proteins at multiple sites can interact with FH 3 domains.
Genes required for initiation and resolution steps of mating-type switching in fission yeast.
The fission yeast Schizosaccharomyces pombe switches mating type by transposition of a copy of DNA derived from either of the two storage cassettes, mat2 -P and mat3 -M, into the expression locus,
Mapping the double‐strand breaks at the mating‐type locus in fission yeast by genomic sequencing.
The technique of genomic sequencing is used to map the position of the break directly on chromosomal DNA cleaved in vivo, finding that the break is situated very close to the right‐hand border of the expressed mat1 cassette.
F-actin distribution and function during sexual differentiation in Schizosaccharomyces pombe.
Treatment with the actin depolymerising drug Latrunculin A showed that F-actin is required for cell fusion and spore formation and the S. pombe homologue of theactin binding protein profilin, Cdc3, was shown to be required for conjugation.
Meiosis in Aspergillus nidulans: another example for lacking synaptonemal complexes in the absence of crossover interference.
This deviation from most other eukaryotes may be of significance for explaining the likewise unusual absence of crossover interference in this organism, as well as in Schizosaccharomyces pombe.
Premeiotic DNA synthesis in fission yeast.
Characterization of fus1 of Schizosaccharomyces pombe: a developmentally controlled function needed for conjugation
Cloned the fus1 gene found that it is capable of encoding a 1,372-amino-acid protein with no significant similarities to other known proteins, and it is concluded that activation of transcription by Ste11 is independent of orientation.
Two-step activation of meiosis by the mat1 locus in Schizosaccharomyces pombe
The results suggest a model in which the mat1 locus plays two roles in controlling meiosis, which appears to be the major pheromone-dependent step in controls meiosis.