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Tomographic 3D Reconstruction of Quick-Frozen, Ca2+-Activated Contracting Insect Flight Muscle
TLDR
Motor actions of myosin were directly visualized by electron tomography of insect flight muscle quick-frozen during contraction, suggesting the MD tilts and slews on actin from weak to strong binding, followed by swinging of the LCD through an approximately 35 degrees axial angle. Expand
Structure of myosin filaments from relaxed Lethocerus flight muscle by cryo-EM at 6 Å resolution
TLDR
A cryo–electron microscopy three-dimensional image reconstruction of relaxed myosin II–containing thick filaments from the flight muscle of the giant water bug Lethocerus indicus is described, revealing unique molecular motor conformation and unprecedented details of the filament backbone. Expand
X-ray diffraction indicates that active cross-bridges bind to actin target zones in insect flight muscle.
TLDR
It is concluded that stretch-activated tension of IFM is produced by cross-bridges that bind to rigor's lead-bridge target zones, comprising < or = 1/3 of the 75-80% that attach in rigor. Expand
The Prohormone VGF Regulates β Cell Function via Insulin Secretory Granule Biogenesis.
TLDR
The data support a model in which VGF operates at a critical node of granule biogenesis in the islet β cell to coordinate insulin biosynthesis with β cell secretory capacity. Expand
Coupling between myosin head conformation and the thick filament backbone structure.
TLDR
This work shows the first structural evidence that the arrangement of myosin heads within the interacting heads motif is coupled to the structure of the thick filament backbone, and suggests a mechanism for how tension affects the dynamics of the myOSin heads. Expand
Cardiac morphology after conditions of microgravity during COSMOS 2044.
TLDR
General morphological features similar to those in ventricular samples from the previous COSMOS 1887 flight were observed and mitochondrial volume density values and mitochondria-to-myofibril ratios as well as normal A-band spacings of myofibrils were observed in the tail-suspension and flight groups. Expand
The Z-band lattice in skeletal muscle in rigor.
TLDR
It appears that the small square form of the Z-band is found in physiological states with little cross-bridge binding and the basketweave form is found for muscles in rigor in states with a high degree of cross- bridge binding. Expand
Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy
TLDR
This review covers the history of this effort and the progress toward visualizing the complex set of conformational changes that myosin heads make when binding to actin in several static states, as well as the fast frozen actively contracting muscle. Expand
Myosin II sequences for Lethocerusindicus
TLDR
A lack of negative charge in the myosin S2 domain may explain why Lethocerus thick filaments display a perpendicular interacting heads motif, rather than one folded back to contact S2, as is seen in other thick filament types such as those from tarantula. Expand
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