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Plasmid-Encoded Phthalate Catabolic Pathway in Arthrobacter keyseri 12B
  • R. Eaton
  • Medicine, Biology
  • Journal of bacteriology
  • 15 June 2001
TLDR
Activities of the eight enzymes involved in the catabolism of phthalate through protocatechuate to pyruvate and oxaloacetate were demonstrated in cells or cell extracts of recombinant E. coli strains. Expand
p-Cymene catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA encoding conversion of p-cymene to p-cumate
  • R. Eaton
  • Medicine, Biology
  • Journal of bacteriology
  • 1 May 1997
TLDR
It was demonstrated that cymR encodes a repressor which controls expression of both the cym and cmt operons and is inducible by p-cumate but not p-cymene. Expand
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon
  • R. Eaton
  • Biology, Medicine
  • Journal of bacteriology
  • 1 March 1996
TLDR
By examining enzyme activities in recombinant bacteria carrying these fragments and sub-cloned fragments, genes encoding most of the enzymes of the p-cumate pathway were located andRecombinant bacteriacarrying certain restriction fragments bordering the cmt operon were found to transform indole to indigo. Expand
Cloning and analysis of s-triazine catabolic genes from Pseudomonas sp. strain NRRLB-12227
TLDR
Restriction mapping and Southern hybridization analyses of these cloned DNA fragments suggested that these s-triazine catabolic genes may be located on a transposable element, the ends of which are identical 2.2-kb insertion sequences. Expand
Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions
TLDR
The reactions involved in the bacterial metabolism of naphthalene to salicylate have been reinvestigated by using recombinant bacteria carrying genes cloned from plasmid NAH7, and the gene order for the nah operon was shown to be p, A, B, F, C, E, D. Expand
Biotransformation of benzothiophene by isopropylbenzene-degrading bacteria
TLDR
Data indicate that oxidation of benzothiophene by strain RE204 is initiated at either ring, and it is proposed that 2,3 dioxygenation yields a product that is both a cis-dihydrodiol and a thiohemiacetal. Expand
Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the
  • R. Eaton
  • Biology, Medicine
  • Journal of bacteriology
  • 1 December 1994
TLDR
The sequence of a 2,437-bp DNA segment from the naphthalene upper catabolic pathway operon of plasmid NAH7 was determined, and it was determined that these directly repeated 84-bp sequences encompass the upper-pathway nah operon and constitute the ends of a highly conserved region. Expand
Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B
TLDR
One of the nonrevertible mutants, strain 12B-Cl, lacked all of the enzymes required for the metabolism of dibutylphthalate through the protocatechuate meta-cleavage pathway, and was given clones at high frequency that had lost the ability to grow with phthalate esters. Expand
Characterization of a plasmid-specified pathway for catabolism of isopropylbenzene in Pseudomonas putida RE204
TLDR
A Pseudomonas putida strain designated RE204, able to utilize isopropylbenzene as the sole carbon and energy source, was isolated and a metabolic pathway is proposed that combines Tn5 transposon insertion, restriction enzyme cleavage, and catabolic enzymes. Expand
Oxidation of naphthenoaromatic and methyl-substituted aromatic compounds by naphthalene 1,2-dioxygenase
TLDR
The action of naphthalene dioxygenase on a variety of methyl-substituted aromatic compounds resulted in the formation of benzylic alcohols, i.e., methyl group monooxygenation products, which were subsequently converted to the corresponding carboxylic acids by dehydrogenase(s) in the host strain. Expand
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