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Nitrous Oxide Reductase
(N2O) is a major greenhouse gas and is thought to be some 300 times more potent than CO2 in promoting global warming due to its inert chemical nature. The difficult chemistry of N2O reduction toExpand
Unprecedented proximal binding of nitric oxide to heme: implications for guanylate cyclase
TLDR
The 1.95 and 1.35 Å resolution crystal structures of the CO‐ and NO‐bound forms of the reduced protein from Alcaligenes xylosoxidans have potential implications for the activation of sGC induced by the binding of NO or CO to the heme domain. Expand
Genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases
TLDR
Genome analysis suggests the widespread occurrence of these novel NiRs with representatives in the α, β and γ subclasses of the Proteobacteria and in two species of the fungus Aspergillus. Expand
Molybdenum and vanadium nitrogenases of Azotobacter chroococcum. Low temperature favours N2 reduction by vanadium nitrogenase.
TLDR
Activity cross-reactions between nitrogenase components showed the enhanced low-temperature activity to be associated with the Fe protein of V nitrogenase, even under conditions of high electron flux obtained at a 12-fold molar excess of Fe protein. Expand
Crystallography with online optical and X-ray absorption spectroscopies demonstrates an ordered mechanism in copper nitrite reductase.
TLDR
Crystallography is used, together with online X-ray absorption spectroscopy and optical spectroscopic techniques, to show that X-rays rapidly and selectively photoreduce the T1Cu centre, but that the T2Cu centre does not photoresist directly over a typical crystallographic data collection time, and an 'ordered' mechanism in which electron transfer is gated by binding of nitrite to the T3Cu centre is demonstrated. Expand
pH-dependence for binding a single nitrite ion to each type-2 copper centre in the copper-containing nitrite reductase of Alcaligenes xylosoxidans.
TLDR
The first quantitative characterization of the interaction of NO2(-) with the Cu-containing dissimilatory nitrite reductase (NiR) of Alcaligenes xylosoxidans using steady-state kinetics, equilibrium gel filtration and EPR spectroscopy shows a similar pH-dependence to that of the activity. Expand
X-ray structure of a blue-copper nitrite reductase in two crystal forms. The nature of the copper sites, mode of substrate binding and recognition by redox partner.
TLDR
A striking difference in the overall surface charge distribution between the two sub-groups is provided, providing a neat structural explanation for their different reactivities to pseudoazurin or azurin and supporting the view that electron transfer proceeds via complex formation. Expand
Ethane formation from acetylene as a potential test for vanadium nitrogenase in vivo
Azotobacter chroococcum and A. vinelandii, both obligately aerobic diazotrophic bacteria, have at least two genetically distinct systems for nitrogen fixation1,2. One is the well-characterizedExpand
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