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Regulation of pathways of mRNA destabilization and stabilization.
The level of an mRNA in the cytoplasm represents a balance between the rate at which the mRNA precursor is synthesized in the nucleus and the rates of nuclear RNA processing and export and… Expand
In Vitro Genetic Analysis of the RNA Binding Site of Vigilin, a Multi-KH-Domain Protein
ABSTRACT The function(s) and RNA binding properties of vigilin, a ubiquitous protein with 14 KH domains, remain largely obscure. We recently showed that vigilin is the estrogen-inducible protein in… Expand
Vigilin, a Ubiquitous Protein with 14 K Homology Domains, Is the Estrogen-inducible Vitellogenin mRNA 3′-Untranslated Region-binding Protein*
RNA-binding proteins containing KH domains are widely distributed. One KH domain protein of unknown function, vigilin (also known as the high density lipoprotein-binding protein), contains 14 KH… Expand
Vigilin binding selectively inhibits cleavage of the vitellogenin mRNA 3'-untranslated region by the mRNA endonuclease polysomal ribonuclease 1.
- K. Cunningham, R. Dodson, M. Nagel, D. Shapiro, Daniel R Schoenberg
- Medicine, Biology
- Proceedings of the National Academy of Sciences…
- 7 November 2000
In Xenopus, estrogen induces the stabilization of vitellogenin mRNA and the destabilization of albumin mRNA. These processes correlate with increased polysomal activity of a sequence-selective mRNA… Expand
Nonsteroidal signals originating in the gonads.
The discovery of the various peptide factors in the gonads followed different paths. A number of factors were specifically searched for because of physiological experiments that predicted that an… Expand
Regulation of the α inhibin gene by cyclic adenosine 3′,5′-monophosphate after transfection into rat granulosa cells
Inhibin gene expression in the ovary is stimulated by FSH, which uses cAMP as an intracellular second messenger. To examine further the transcriptional regulation of the α inhibin gene by FSH and… Expand
An estrogen-inducible protein binds specifically to a sequence in the 3' untranslated region of estrogen-stabilized vitellogenin mRNA.
The 3' untranslated region (3'-UTR) has been implicated in the estrogen stabilization of hepatic Xenopus laevis vitellogenin mRNA. We used RNA gel mobility shift assays to demonstrate that Xenopus… Expand
Tissue distribution, hormone regulation and evidence for a human homologue of the estrogen-inducible Xenopus laevis vitellogenin mRNA binding protein
- R. Dodson, M. Acena, D. Shapiro
- Biology, Medicine
- The Journal of Steroid Biochemistry and Molecular…
- 1 June 1995
17 beta-estradiol induces the synthesis of massive amounts of the hepatic mRNA encoding the Xenopus laevis egg yolk precursor protein, vitellogenin. Vitellogenin mRNA exhibits a half life of approx.… Expand
RNA gel shift assays for analysis of hormone control of mRNA stability.
- R. Dodson, Kathryn M Goolsby, Maria Acena-Nagel, C. Mao, D. Shapiro
- Biology, Medicine
- Methods in enzymology
Publisher Summary This chapter focuses on the regulation of the cytoplasmic stability of specific mRNAs. The level of an mRNA in the cytoplasm represents a balance between the rate at which the mRNA… Expand
Analysis of RNA-protein interactions by a microplate-based fluorescence anisotropy assay.
- C. Mao, Kathryn Flavin, S. Wang, R. Dodson, J. Ross, D. Shapiro
- Medicine, Chemistry
- Analytical biochemistry
- 15 March 2006
Quantitative studies of RNA-protein interactions are quite cumbersome using traditional methods. We developed a rapid microplate-based fluorescence anisotropy (FA)/fluorescence polarization assay… Expand