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Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella.
Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells.
Using an in vitro system, a genetic locus is isolated that confers to a noninvasive strain of Salmonella typhimurium the ability to penetrate tissue culture cells and show decreased ability to colonize the Peyer's patches, the small intestinal wall, and the spleen when administered perorally and intraperitoneally.
Reversible Microbial Colonization of Germ-Free Mice Reveals the Dynamics of IgA Immune Responses
A reversible germ-free colonization system in mice that is independent of diet or antibiotic manipulation is reported, and specific IgA induction occurred as a stepwise response to current bacterial exposure, such that the antibody repertoire matched the existing commensal content.
Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Escherichia coli strain
The isolation and characterization of a gene that may be important in the adherence of avian pathogenic Escherichia coli to the avian respiratory tract and the deduced protein was found to contain significant homology to the Haemophilus influenzae and Neisseria gonorrhoeae immunoglobulin A1 proteases are reported.
MlrA, a novel regulator of curli (AgF) and extracellular matrix synthesis by Escherichia coli and Salmonella enterica serovar Typhimurium
- P. K. Brown, C. Dozois, C. Nickerson, A. Zuppardo, J. Terlonge, R. Curtiss
- BiologyMolecular microbiology
- 1 July 2001
Results indicate that MlrA is a newly defined transcriptional regulator of csgD/agfD that acts as a positive regulator of RpoS‐dependent curli and extracellular matrix production by E. coli and S. typhimurium.
Relationship between the Tsh Autotransporter and Pathogenicity of Avian Escherichia coli and Localization and Analysis of the tsh Genetic Region
- C. Dozois, M. Dho-Moulin, A. Brée, J. Fairbrother, C. Desautels, R. Curtiss
- BiologyInfection and Immunity
- 1 July 2000
Results demonstrate that thetsh gene is frequently located on the ColV virulence plasmid in APEC and suggest a possible role of Tsh in the pathogenicity of E. coli for chickens in the early stages of infection.
Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain
- C. Dozois, F. Daigle, R. Curtiss
- BiologyProceedings of the National Academy of Sciences…
- 27 December 2002
The approach used simultaneously provided a means to identify novel pathogen-specific genes expressed in vivo and insight regarding the global gene expression and physiology of a pathogenic E. coli strain in a natural animal host during the infectious process.
Plasmid-associated virulence of Salmonella typhimurium
The role of the 100-kb plasmid of S. typhimurium in virulence is confirmed, primarily in invasion to mesenteric lymph nodes and spleens after peroral inoculation of mice.
Immune Responses to Recombinant Pneumococcal PspA Antigen Delivered by Live Attenuated Salmonella enterica Serovar Typhimurium Vaccine
Oral immunization with Salmonella-PspA vaccine protected 60% of immunized mice from death after intraperitoneal challenge with 50 times the 50% lethal dose of virulent S. pneumoniae WU2.
Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines
- G. Georgiou, C. Stathopoulos, P. Daugherty, A. Nayak, B. Iverson, R. Curtiss
- BiologyNature Biotechnology
Cell displaying protein receptors or antibodies are of use for analytical applications and bioseparations, and Expression of antigens on the surface of nonvirulent microorganisms is an attractive approach to the development of high-efficacy recombinant live vaccines.