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Spider: An R package for the analysis of species identity and evolution, with particular reference to DNA barcoding

Spider: SPecies IDentity and Evolution in R is a new R package implementing a number of useful analyses for DNA barcoding studies and associated research into species delimitation and speciation.…
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The seven deadly sins of DNA barcoding

TLDR
An assessment of seven deficiencies that are common in the DNA barcoding literature are provided, and some potential improvements for its adaptation and adoption towards more reliable and accurate outcomes are outlined.
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Molecular markers for the phylogenetics of mites and ticks

TLDR
This review describes the properties of the ideal molecular marker and compare these with genes that have been used for phylogenetic studies of mites and ticks and suggests some nuclear protein-coding genes that may be suitable alternatives.
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Barcoding's next top model: an evaluation of nucleotide substitution models for specimen identification

TLDR
It is reported that the K2P was a poorly fitting model at the species level; it was never selected as the best model and very rarely selected as a credible alternative model; and simpler metrics such as p distance performed equally well, perhaps obviating the requirement for model correction in DNA barcoding.
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Phylogenetic analysis of partial sequences of elongation factor 1alpha identifies major groups of lice (Insecta: Phthiraptera).

TLDR
Evidence is provided in support of the hypothesis that lice have speciated in situ on the host in response to niche specialization and that this has given rise to convergent morphologies in the lice of different host groups which share similar ecological niches.
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Barcoding and Border Biosecurity: Identifying Cyprinid Fishes in the Aquarium Trade

TLDR
It is demonstrated that DNA barcoding provides a highly effective biosecurity tool for rapidly identifying high-risk fishes in the aquarium trade, and is improved on previous studies by consolidating supplementary information from multiple data sources.
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Dramatically elevated rate of mitochondrial substitution in lice (Insecta: Phthiraptera).

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Phylogenetic analysis of the mitochondrial cytochrome oxidase subunit I gene of five species of the Culicoides imicola species complex

TLDR
Phylogenetic trees constructed using neighbour‐ joining, parsimony and maximum likelihood showed each species to be distinct and Combinations of sites for two restriction enzymes in the COI sequences were species‐specific and could form the basis of a diagnostic PCR assay.
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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

TLDR
A standardised set of procedures for use on different taxa and sample media is proposed, largely based on recent trends in their use, and represents a framework for efficient, comprehensive and robust DNA-based investigations of biodiversity, applicable to mostTaxa and ecosystems.
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Phylogenetic status and matrilineal structure of the biting midge, Culicoides imicola, in Portugal, Rhodes and Israel

TLDR
The results provide phylogenetic evidence that C. imicola in the study areas are potentially competent AHS and BT vectors, and suggest that population subdivision in its vector can impose spatial constraints on BT virus transmission.
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