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Insights from retinitis pigmentosa into the roles of isocitrate dehydrogenases in the Krebs cycle
Findings support the hypothesis that mitochondrial NADP-IDsH, rather than NAD-IDH, serves as the main catalyst for this reaction in the citric acid cycle outside the retina, and that the retina has a particular requirement for NAD- IDH. Expand
Crystal Structure of Porcine Mitochondrial NADP+-dependent Isocitrate Dehydrogenase Complexed with Mn2+ and Isocitrate
The crystal structure of porcine heart mitochondrial NADP+-dependent isocitrate dehydrogenase (IDH) complexed with Mn2+ and isOCitrate was solved to a resolution of 1.85 Å by multiwavelength anomalous diffraction phasing using selenium substitution in the form of selenomethionine as the anomalous scatterer. Expand
Direct evidence for the formation of a complex between 1-cysteine peroxiredoxin and glutathione S-transferase pi with activity changes in both enzymes.
It is demonstrated that a heterodimer complex is formed between 1-Cys Prx with a C-terminal His6 tag and GST pi upon incubation of the two proteins at pH 8.0, followed by dialysis against buffer containing 2.5 mM glutathione (GSH) but lacking 1,6-hexanediol. Expand
Affinity labeling of purine nucleotide sites in proteins.
  • R. Colman
  • Chemistry, Medicine
  • Annual review of biochemistry
  • 1983
Adenylosuccinate lyase deficiency.
Both the presence of normal levels of ADSL enzyme activities in some patient tissues and the absence of a clear correlation between mutations, biochemistry, and behavior show that the system has unexplored biochemical and/or genetic complexity. Expand
Chemical characterization of distinct subunits of pig heart DPN-specific isocitrate dehydrogenase.
The results suggest that a complete DPN-specific isocitrate dehydrogenase would have a minimum molecular weight of 160,000, which is much simpler than that of the total enzyme. Expand
Clopidogrel inhibits the binding of ADP analogues to the receptor mediating inhibition of platelet adenylate cyclase.
Clopidogrel, like the homologous thienopyridine derivative ticlopidine, selectively inhibits platelet aggregation induced by ADP. We have previously described two nucleotide-binding sites onExpand
Substrate and product complexes of Escherichia coli adenylosuccinate lyase provide new insights into the enzymatic mechanism.
Structural alignments of ec-ADL with other superfamily members suggest for the first time a large conformational movement of the flexible C3 loop in ec- ADL upon substrate binding and catalysis, resulting in its closure over the active site. Expand
Critical Role of Lys212 and Tyr140 in Porcine NADP-dependent Isocitrate Dehydrogenase*
The results suggest that in wild type enzyme, Tyr140 is the general acid that protonates the substrate after decarboxylation and that the carboxyl and ammonium forms of Y140E and Y140K provide partial substitutes. Expand
Expression, purification, and characterization of stable, recombinant human adenylosuccinate lyase.
This is the first purification procedure to yield a stable form of human adenylosuccinate lyase and it is revealed that the histidine-tagged enzyme is in tetrameric form with a molecular weight of 220 kDa and contains predominantly alpha-helical structure. Expand