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Sirt5 Is a NAD-Dependent Protein Lysine Demalonylase and Desuccinylase
It is found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro and may represent a posttranslational modification that can be reversed by Sirt 5 in vivo. Expand
Structure of the Rho Family GTP-Binding Protein Cdc42 in Complex with the Multifunctional Regulator RhoGDI
The structural data demonstrate how GDIs serve as negative regulators of small GTP-binding proteins and how the isoprenoid moiety is utilized in this critical regulatory interaction. Expand
Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3.
It is demonstrated that Cdc24 and Bem3 have GDP-release factor activity and GTPase-activating protein activity, respectively, toward the essential bud-site assembly GTP enzyme Cdc42, and a new gene is reported that is a multicopy suppressor of the temperature-sensitive lethality caused by mutations in the bud emergence gene BEM2. Expand
Cdc42 and PAK-mediated Signaling Leads to Jun Kinase and p38 Mitogen-activated Protein Kinase Activation (*)
The PAK family of protein kinases has been suggested as a potential target of the Cdc42 and Rac GTPases based on studies in vitro but it is shown that PAK-3 is activated by CDC42 in vivo and may mediate the effects of cytokines on transcriptional regulation. Expand
Interactions between the bud emergence proteins Bem1p and Bem2p and Rho- type GTPases in yeast
The SH3 domain-containing protein Bem1p is needed for normal bud emergence and mating projection formation, two processes that require asymmetric reorganizations of the cortical cytoskeleton inExpand
Structural basis for the guanine nucleotide-binding activity of tissue transglutaminase and its regulation of transamidation activity
The structure suggests a structural basis for the negative regulation of transamidation activity by bound nucleotide, and the positive regulation oftransamidation by Ca2+. Expand
A Novel Regulator of p21-activated Kinases*
The identification of two closely related Pak3-binding proteins, possibly arising from alternative splicing, designated p50 and p85Cool-1 (cloned out of library) are reported, indicating a novel mechanism of regulation of Pak signaling. Expand
Identification of a Mouse p21Cdc42/Rac Activated Kinase (*)
It is found that mPAK-3, expressed in vitro and in vivo, shows highly specific binding to the SH3 domain of phospholipase C- and at least one SH3domain in the adapter protein Nck, raising the possibility of an additional level of regulation of the PAK family in vivo. Expand
The Dbl family of oncogenes.
There is still a good deal to learn regarding the biochemical mechanisms that underlie the Dbl family of proteins, and these positive regulators of G proteins to specific cellular locations to carry out the signaling task. Expand
Targeting mitochondrial glutaminase activity inhibits oncogenic transformation.
A small molecule inhibitor is described that blocks oncogenic transformation induced by various Rho GTPases in fibroblasts, and the growth of human breast cancer and B lymphoma cells, without affecting normal cells. Expand