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Occurrence of the regulatory nucleotides ppGpp and pppGpp following induction of the stringent response in staphylococci.
The stringent response in Escherichia coli and many other organisms is regulated by the nucleotides ppGpp and pppGpp. We show here for the first time that at least six staphylococcal species alsoExpand
Evidence that the immediate biosynthetic precursor of clavulanic acid is its N-aldehyde analogue
(3R, 5R) Clavulanate-9-aldehyde 1 has been detected in Streptomyces clavuligerus and an NADPH dependent dehydrogenase capable of reducing 1 to clavulanic acid 2 has been isolated from this organism.
SB 205952, a novel semisynthetic monic acid analog with at least two modes of action.
The biological properties of SB 205952, a nitrofuryl oxazole derivative of monic acid, differ from those of the closely related antibacterial agent mupirocin. Compared with mupirocin, SB 205952 hasExpand
Study of the tryptophan residues of lysozyme using 1H nuclear magnetic resonance.
The identification and complete assignment of the C-2 and N-1 proton nuclear magnetic resonances (NMR) of the six tryptophan residues of hen lysozyme are reported. Identification of the resonancesExpand
Structure and binding properties of hen lysozyme modified at tryptophan 62.
Abstract The structure of a derivative of hen egg-white lysozyme (EC modified by N -bromosuccinimide at Trp62 has been studied by both 1 H nuclear magnetic resonance spectroscopy and X-rayExpand
The interaction of streptokinase.plasminogen activator complex, tissue-type plasminogen activator, urokinase and their acylated derivatives with fibrin and cyanogen bromide digest of fibrinogen.
The effects of purified soluble fibrin and of fibrinogen fragments (fibrin mimic) on the activation of Lys-plasminogen (i.e. plasminogen residues 77-790) to plasmin by streptokinase.plasminogenExpand
The Chemistry of Pseudomonic Acid.† 18. Heterocyclic Replacement of the α,β-Unsaturated Ester: Synthesis, Molecular Modeling, and Antibacterial Activity1
The electronic requirements around the C1-C3 region of pseudomonic acid analogues were investigated. Synthetic routes were developed to access a range of compounds where the alpha, beta-unsaturatedExpand
Isolation of dihydroclavaminic acid, an intermediate in the biosynthesis of clavulanic acid
Abstract A primary isotope effect was utilised in an in vitro study to allow the isolation and characterisation of an intermediate between proclavaminic acid and clavaminic acid, in clavulanic acidExpand
Expression in Escherichia coli of a clavaminic acid synthase isozyme : a trifunctional oxygenase involved in clavulanic acid biosynthesis
Abstract Purification and sequencing of clavaminic acid synthase (CAS) activities from S. clavuligerus SC2 provided evidence for the existence of two CAS isozymes. One of these isozymes was clonedExpand
SB-203207 and SB-203208, two novel isoleucyl tRNA synthetase inhibitors from a Streptomyces sp. I. Fermentation, isolation and properties.
Two novel inhibitors of isoleucyl tRNA synthetase designated SB-203207 and SB-203208 have been detected in the culture of a new Streptomyces species. The fermentation, isolation and some propertiesExpand