• Publications
  • Influence
Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries
We constructed a single-chain Fv antibody library that permits human complementarity-determining region (CDR) gene fragments of any germline to be incorporated combinatorially into the appropriate
Recombinant Human Antibodies Against Aldehyde-Modified Apolipoprotein B-100 Peptide Sequences Inhibit Atherosclerosis
These findings suggest that antibodies are important mediators of atheroprotective immune responses directed to oxidized LDL, and passive immunization against MDA-modified apoB-100 peptide sequences may represent a novel therapeutic approach for prevention and treatment of cardiovascular disease.
Telomerase activation in normal B lymphocytes and non-Hodgkin's lymphomas.
The data suggest that telomerase activity in malignant lymphomas can be explained by an "induction and retention" model, ie, transformation occurs in a normal, mature B cell with reactivated telomersase, which is retained in the neoplastic clone.
Binding of staphylococcal enterotoxin A to accessory cells is a requirement for its ability to activate human T cells.
Staphylococcal enterotoxin A (SEA) activates human T cells at extremely low concentrations corresponding to 1 to 5 molecules/T cell. SEA, in contrast to other polyclonal activators, is absolutely
Laminin and fibronectin in cell adhesion: enhanced adhesion of cells from regenerating liver to laminin.
The increase of laminin in regenerating liver and the adhesiveness of cells from such livers to lamin in suggest a role for laminIn in the maintenance of a proper tissue organization during liver regeneration.
Co-ligation of CD44 on naive human tonsillar B cells induces progression towards a germinal center phenotype.
It is shown that an additional co-ligation of the glycoprotein CD44 on resting human tonsillar B cells up-regulated the typical human GC markers CD10, CD38, CD77 and CD95, and down-regulated CD24 and CD39 as well as induced progression towards apoptosis in these cells; all characteristics of GC B cells.
Binding of staphylococcal enterotoxin A to HLA-DR on B cell lines.
The results show that the HLA-DR molecule is the main functional molecule for binding ofSEA to accessory cells and that this binding of SEA to Hla-DR is a necessary requirement for SEA-induced T cell activation.
Human CD4+ T cells expressing CD45RA acquire the lymphokine gene expression of CD45RO+ T-helper cells after activation in vitro.
The results indicate that theCD45RA subset is a precursor of the CD45RO and further strengthen the hypothesis that the former cell population represents naive whereas the latter subset represents memory T cells within the CD4 subset.
Automated screening procedure for high-throughput generation of antibody fragments.
The automated system is configured to screen tens of thousands of clones per day to target antigens in various formats, including peptides and soluble proteins, as well as cell-bound targets; thus, it is well designed to meet demands from the proteomics area.
Telomerase regulation and telomere dynamics in germinal centers
The unique telomere elongation in GC B cells permits extensive proliferation during the GC reaction and provides the memory cells with a substantial increase in division potential.