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Noncanonical Inflammasome Activation by Intracellular LPS Independent of TLR4
It is shown that macrophages loaded with synthetic lipid A, E. coli lipopolysaccharide (LPS), or S. typhimurium LPS activate caspase-11 independently of the LPS receptor Toll-like receptor 4 (TLR4), revealing a TLR4-independent mechanism for innate immune recognition of LPS. Expand
Identification of an Orphan Response Regulator Required for the Virulence of Francisella spp. and Transcription of Pathogenicity Island Genes
DNA microarray analysis identified 65 genes regulated by PmrA, which is an important factor in controlling virulence in F. novicida, and a pmrA mutant strain is an effective vaccine against homologous challenge. Expand
Differential Induction of the Toll-Like Receptor 4-MyD88-Dependent and -Independent Signaling Pathways by Endotoxins
Neisseria meningitidis endotoxin was a potent agonist of both the MyD88-dependent and -independent signaling pathways of the TLR4 receptor complex of human macrophages and response was restored inTLR4-MD-2-transfected human embryonic kidney 293 cells. Expand
Structural Characterization of the Pseudomonas aeruginosa 1244 Pilin Glycan*
An antigenic similarity between lipopolysaccharide (LPS) and glycosylated pilin of Pseudomonas aeruginosa 1244 was noted. We purified a glycan-containing molecule from proteolytically digested piliExpand
Receptor-mediated exopolysaccharide perception controls bacterial infection
It is shown that Epr3 expression is inducible and dependent on host perception of bacterial nodulation (Nod) factors, and that plant–bacterial compatibility and bacterial access to legume roots is regulated by a two-stage mechanism involving sequential receptor-mediated recognition of Nod factor and EPS signals. Expand
A 2-O-methylfucose moiety is present in the lipo-oligosaccharide nodulation signal of Bradyrhizobium japonicum.
Comparative analysis of the B. japonicum Nod metabolite and those characterized from Rhizobium species suggests that the presence of the fucosyl residue plays an important role in the specificity of the H2O-soybean symbiosis. Expand
The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin.
The crystal structure of the soluble domain of the protein of the lipid A PEA transferase from Neisseria meningitidis has been determined crystallographically and refined to 1.4Å resolution and reveals the protein components needed for the transferase chemistry; however, substrate-binding regions are not evident and are likely to reside in the transmembrane domain ofthe protein. Expand
Rhizobium fredii and Rhizobium meliloti produce 3-deoxy-D-manno-2-octulosonic acid-containing polysaccharides that are structurally analogous to group II K antigens (capsular polysaccharides) found
Polyacrylamide gel electrophoresis, nuclear magnetic resonance spectrometry, and gas chromatography analyses showed that both of these bacterial species produce unique polysaccharides that contain a high proportion of 3-deoxy-D-manno-2-octulosonic acid (Kdo). Expand
Lipopolysaccharides and K-Antigens: Their Structures, Biosynthesis, and Functions
The bacterial surface is the first line of defense against antimicrobial molecules and stress caused by changes in the environment surrounding the bacterium. In the case of plant- and animal-microbeExpand
A novel polar surface polysaccharide from Rhizobium leguminosarum binds host plant lectin
In isolation and partial characterization of a novel high molecular weight surface polysaccharide from a strain of Rhizobium leguminosarum that nodulates Pisum sativum and Vicia sativa roots, data are consistent with the hypothesis that rhizobia can use (at least) two mechanisms for docking at the root surface, with use of a lectin–glycan mechanism under slightly acidic conditions. Expand