• Publications
  • Influence
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
TLDR
The latest red version matures more completely, is more tolerant of N-terminal fusions and is over tenfold more photostable than mRFP1, and three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies.
A monomeric red fluorescent protein
TLDR
This work presents the stepwise evolution of DsRed to a dimer and then either to a genetic fusion of two copies of the protein, i.e., a tandem dimer, or to a true monomer designated mRFP1 (monomeric red fluorescent protein).
An Expanded Palette of Genetically Encoded Ca2+ Indicators
TLDR
This series enables improved single-color Ca2+ imaging in neurons and transgenic Caenorhabditis elegans and develops blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change.
Reducing the Environmental Sensitivity of Yellow Fluorescent Protein
TLDR
Citrine is superior to all previous YFPs except when pH or halide sensitivity is desired and is particularly advantageous within genetically encoded fluorescent indicators of physiological signals.
New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.
TLDR
Affinities in vitro and detection limits in living cells are optimized with Xaa-Xaa = Pro-Gly, suggesting that the preferred peptide conformation is a hairpin rather than the previously proposed alpha-helix.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
TLDR
The Optopatch platform enables high-throughput, spatially resolved electrophysiology without the use of conventional electrodes and revealed homeostatic tuning of intrinsic excitability in human stem cell–derived neurons.
Creating new fluorescent probes for cell biology
TLDR
The address list shown was incorrect and should have read as above and the address of the author should have reading as above.
Creating new fluorescent probes for cell biology
TLDR
Advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein–protein interactions.
Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.
TLDR
The engineering of the first monomeric version of the tetrameric CFP (cyan FP) cFP484 from Clavularia coral is reported, named mTFP1 [monomeric TFP1 (teal FP 1], one of the brightest and most photostable FPs reported to date.
Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins.
TLDR
Improved versions of BFP are reported along with a variety of engineered fluorescent protein variants with novel and distinct chromophore structures that all share the property of a blue fluorescent hue.
...
...