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A rapid filtration assay for soluble receptors using polyethylenimine-treated filters.
Ligand bound to detergent-solubilized or cytosolic receptors can be separated from free ligand by filtration through glass-fiber filters which have been pretreated with polyethylenimine (PEI).Expand
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Characterization of the A2 adenosine receptor labeled by [3H]NECA in rat striatal membranes.
[3H]NECA (1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-beta-D-ribofuronamide) is known to bind to both the A1 and A2 subtypes of adenosine receptor in rat striatal membranes. In order to study theExpand
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Allosteric enhancement of adenosine A1 receptor binding and function by 2-amino-3-benzoylthiophenes.
Several 2-amino-3-benzoylthiophenes were found to increase the binding of [3H]N6-cyclohexyladenosine to A1 adenosine receptors in rat brain membranes. Concentration-response curves were bell-shaped,Expand
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Binding of the A1-selective adenosine antagonist 8-cyclopentyl-1,3-dipropylxanthine to rat brain membranes
Summary8-Cyclopentyl-1,3-dipropylxanthine (PD 116,948) is a very potent, very A1-selective adenosine antagonist, with a Ki of 0.46 nM in 3H-CHA binding to A1 receptors in rat whole brain membranesExpand
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Adenosine receptors and behavioral actions of methylxanthines.
Central stimulant actions of 10 methylxanthines in mice correlate with affinities for adenosine receptors labeled with N6-[3H]cyclohexyladenosine. Affinities of methylxanthines for adenosineExpand
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  • Open Access
Subclasses of angiotensin II binding sites and their functional significance.
Specific binding sites for angiotensin II were identified in the rabbit adrenal gland and uterus and in the rat liver and were divided into two subclasses based on inhibition by nonpeptide ligands.Expand
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Adenosine receptors in brain membranes: binding of N6-cyclohexyl[3H]adenosine and 1,3-diethyl-8-[3H]phenylxanthine.
N6-Cyclohexyl[3H]adenosine ([3H]CHA) and 1,3-diethyl-8-[3H]phenylxanthine ([3H]DPX) to bind to adenosine receptors in brain membranes. The agonist [3H]CHA has high affinity in both bovine and guineaExpand
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Inhibition of protein kinase C by calphostin C is light-dependent.
Calphostin C, a secondary metabolite of the fungus Cladosporium cladosporioides, inhibits protein kinase C by competing at the binding site for diacylglycerol and phorbol esters. Calphostin C is aExpand
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Rules for identifying potentially reactive or promiscuous compounds.
This article describes a set of 275 rules, developed over an 18-year period, used to identify compounds that may interfere with biological assays, allowing their removal from screening sets. ReasonsExpand
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Adenosine receptors in the central nervous system: relationship to the central actions of methylxanthines.
Adenosine has a significant role in many functions of the central nervous system. Behaviorally, adenosine and adenosine analogs have marked depressant effects. Electrophysiologically, adenosineExpand
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