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The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.
TLDR
The structural results give new insight into the physical basis of the remarkable sequence specificity of this enzyme. Expand
A simple DNase model system comprising a dinuclear Zn(II) complex in methanol accelerates the cleavage of a series of methyl aryl phosphate diesters by 10(11)-10(13).
TLDR
The data are consistent with a two step mechanism for the catalyzed reaction with rate limiting formation of a catalyst-coordinated phosphorane intermediate, followed by fast loss of the aryloxy leaving group. Expand
The dinuclear Zn(II) complex catalyzed cyclization of a series of 2-hydroxypropyl aryl phosphate RNA models: progressive change in mechanism from rate-limiting P-O bond cleavage to substrate binding.
TLDR
The pronounced catalytic activity is attributed to a synergism between a positively charged catalyst that has high affinity for the substrate and for the transition state for cyclization, and a medium effect involving a reduced polarity/dielectric constant that complements a reaction where an oppositely charged reactant and catalyst experience charge dispersal in the Transition state. Expand
DFT computational study of the methanolytic cleavage of DNA and RNA phosphodiester models promoted by the dinuclear Zn(II) complex of 1,3-bis(1,5,9-triazacyclododec-1-yl)propane.
TLDR
A density functional theory study of the cleavage of a DNA model and two RNA models promoted by the dinuclear Zn((II)) complex of 1,3-bis(1,5,9-triazacyclodododec-1-yl)propane formulated with a bridging methoxide was undertaken to determine possible mechanisms for the transesterification processes that are consistent with experimental data. Expand
Investigation of the effect of oxy bridging groups in dinuclear Zn(II) complexes that catalyze the cleavage of a simple phosphate diester RNA analogue.
TLDR
The results indicate that the presence of a bridging oxyanion ligand between the metal centers, a common structural element required for the successful formation of many small molecule dinuclear catalysts that show cooperative activity in water, significantly impairs the catalytic efficiency for cleavage of 2. Expand
Enzyme-like acceleration for the hydrolysis of a DNA model promoted by a dinuclear Zn(II) catalyst in dilute aqueous ethanol.
TLDR
Analysis of the rates and products of cleavage of methyl (2-chloro-4-nitrophenyl) phosphate promoted by a dinuclear Zn(II) complex and kinetics of the reaction at 28 mM water suggest that complex 3 promotes the hydrolysis at least 1000 times more effectively than ethanolysis. Expand
An ortho-palladated dimethylbenzylamine complex as a highly efficient turnover catalyst for the decomposition of P=S insecticides. Mechanistic studies of the methanolysis of some P=S-containing
TLDR
An associative mechanism for the catalyzed methanolysis of the P=S pesticides is proposed where a transiently coordinated S=P substrate is intramolecularly attacked by the Pd(II)-coordinated methoxide. Expand
Characterization of the active site and insight into the binding mode of the anti-angiogenesis agent fumagillin to the manganese(II)-loaded methionyl aminopeptidase from Escherichia coli
TLDR
The inhibitory action of this anti-angiogenesis agent on EcMetAP-I appears to involve covalent binding to a polypeptide component at or near the active site rather than direct binding to the metal ions. Expand
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