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Geminivirus strain demarcation and nomenclature
The status of geminivirus species and strain demarcation is reviewed as well as providing updated isolate descriptors for a total of 672 begomovirus isolates, and several others previously classified as “strains” have been upgraded to “species”. Expand
Identification of dna components required for induction of cotton leaf curl disease.
A single-stranded DNA molecule approximately 1350 nucleotides in length is identified which, when coinoculated with the begomovirus to cotton, induces symptoms typical of CLCuD, including vein swelling, vein darkening, leaf curling, and enations. Expand
A unique virus complex causes Ageratum yellow vein disease.
It is demonstrated that yellow vein disease of A. conyzoides results from co-infection by AYVV DNA A and a circular DNA that is approximately half its size that is designated DNA beta, which represents a satellite-like DNA. Expand
Diversity of DNA beta, a satellite molecule associated with some monopartite begomoviruses.
Analysis of sequences revealed a highly conserved organization for DNA beta molecules consisting of a single conserved open reading frame, an adenine-rich region, and a region of high sequence conservation [the satellite conserved region (SCR). Expand
Characterisation of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: evidence for acquisition of a DNA B component by a monopartite begomovirus.
SLCMV DNA A has biological characteristics of a monopartite begomovirus, and the virus probably evolved by acquisition of a DNA B component from ICMV, which was transmitted with low efficiency from cassava to Nicotiana benthamiana. Expand
Geminivirus transmission and biological characterisation of Bemisia tabaci (Gennadius) biotypes from different geographic regions
The recently identified “B” biotype, characterised by a diagnostic esterase banding pattern and by its ability to induce phytotoxic responses in squash, honeysuckle and nightshade was readily distinguished from non-’B’ biotype populations. Expand
Revision of Begomovirus taxonomy based on pairwise sequence comparisons
Revised guidelines for the classification and nomenclature of begomoviruses are proposed and genome-wide pairwise identities of 91 % and 94 % are proposed as the demarcation threshold for begomviruses belonging to different species and strains, respectively. Expand
Geminivirus disease complexes: an emerging threat.
Small circular single-stranded DNA satellites have recently been isolated from plants infected with whitefly-transmitted monopartite begomoviruses, suggesting that these disease complexes pose a serious threat to tropical and sub-tropical agro-ecosystems worldwide. Expand
Virology division news: Revision of taxonomic criteria for species demarcation in the family Geminiviridae, and an updated list of begomovirus species
Members of the family Geminiviridae characteristically have circular single-stranded DNAgenomes packaged within twinned (so-called geminate) particles that cause yield losses to many crop plants throughout the world. Expand
Universal primers for the PCR-mediated amplification of DNA β
DNA β is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helperExpand