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A polymerase chain reaction method adapted for selective amplification and cloning of 3' sequences of potyviral genomes: application to dasheen mosaic virus.
'Universal' degenerate oligonucleotide primers were used to amplify cDNA sequences containing the 3' untranslated region (3' UTR) and a portion of the coat protein gene sequence of dasheen mosaicExpand
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Frequencies and types of exchange aberrations induced by X-rays and neutrons in Chinese hamster splenocytes detected by FISH using chromosome-specific DNA libraries.
PURPOSE To estimate the frequencies of radiation- (low and high LET) induced chromosome aberrations in Chinese hamster splenocytes by two-colour fluorescence in situ hybridization using DNA paintingExpand
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Testis-specific β2 tubulins are identical in Drosophila melanogaster and D. hydei but differ from the ubiquitous β1 tubulin
In Drosophila as in many organisms β tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the β1 and β2 tubulins of Drosophila melanogaster and theExpand
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Cloning, sequencing, and expression in Escherichia coli of the coat protein gene of a new potyvirus infection South African Passiflora.
Complementary DNA representing 1418 nucleotides (nt) of the 3'-poly(A)-proximal tract of the genomic RNA of a potyvirus causing woodiness disease in South African passion fruit, was cloned andExpand
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Testis-specific beta 2 tubulins are identical in Drosophila melanogaster and D. hydei but differ from the ubiquitous beta 1 tubulin.
In Drosophila as in many organisms beta tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the beta 1 and beta 2 tubulins of DrosophilaExpand
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Transcription of repetitive DNA sequences in the lampbrush loop pair Nooses formed by sterile alleles of fertility gene Q on the Y chromosome of Drosophila hydei
The Y chromosomal lampbrush loop-forming male fertility genes of Drosophila consist mainly of repetitive DNA sequences that do not code for proteins. We investigated whether differences in theExpand
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