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Characterization of two maize HSP90 heat shock protein genes: expression during heat shock, embryogenesis, and pollen development.
Southern blot analysis and hybrid select translations indicate that the highly homologous hsp82 and hsp81 genes are members of a small multigene family composed of at least two and perhaps three or more gene family members.
A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean
- C. M. Hernandez-Garcia, A. Martinelli, R. Bouchard, J. Finer
- BiologyPlant Cell Reports
- 20 February 2009
The Gmubi promoter showed high levels of constitutive expression and represents an alternative to viral promoters for driving gene expression in soybean as well as in stably transformed soybean tissues.
The independent stage-specific expression of the 18-kDa heat shock protein genes during microsporogenesis in Zea mays L.
These observations imply that the stage-specific expression of particular 18-kDa hsp genes results from gene-specific regulation during microsporogenesis and gametophyte development rather than from an overall activation of the heat shock or stress response.
Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system
A novel automated image collection and analysis system was used to compare two new soybean (Glycine max (L.) Merr.) promoters with the cauliflower mosaic virus 35S (CaMV35S) promoter, which was used…
Moderately repetitive DNA in evolution.
- R. Bouchard
- BiologyInternational review of cytology
High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline
expression intensity data is presented on 20 novel soybean promoters from two different gene families, ubiquitin and ERF and the utility of lima bean cotyledons and soybean hairy roots for rapid promoter analyses is demonstrated and novel insights are provided towards the utilization of these expression systems.
Isolation and characterization of a small heat shock protein gene from maize.
It is concluded that this maize genomic DNA clone, recovered through its homology to clones for meiotic transcripts in lily, represents a genuine maize small heat shock protein gene.
Complete sequence and development of a full-length infectious clone of an Ohio isolate of Maize dwarf mosaic virus (MDMV).
Cloning and characterization of NSP1, a locus encoding a component of a CDC25‐dependent, nutrient‐responsive pathway in Saccharomyces cerevisiae
Phosphoprotein analysis of NSP1‐suppressed cells indicates that theNSP1 product controls the phosphorylation of two 31 kD proteins whose phosphorylated and de‐phosphorylation are strongly correlated with cell‐cycle arrest and proliferation, respectively, and suggests that the N SP1 product is an important downstream element of a CDC25‐dependent, nutrient‐responsive, phosphorylate pathway.
General recombination mechanisms in extracts of meiotic cells
The occurrence of peak levels of m-rec and recombination activity in extracts from cells in early pachytene points strongly to that stage as the time at which the enzymatic phase of recombination occurs.