Macrophages synthesise and release prostaglandins in response to inflammatory stimuli
It is shown that macrophages whose phospholipid components were labelled with3H-arachidonic acid also synthesise and release 3H-prostaglandins (PGs) in response to inflammatory stimuli, consistent with the findings that human Macrophages on intrauterine devices and guinea pig macrophage responding to lymphokines release PGs.
Regulation of prostaglandin synthesis and of the selective release of lysosomal hydrolases by mouse peritoneal macrophages.
- R. Bonney, P. Wightman, P. Davies, S. Sadowski, F. Kuehl, J. Humes
- Biology, MedicineBiochemical Journal
- 15 November 1978
The zymosan-stimulated synthesis and release of prostaglandins are completely inhibited by indomethacin, whereas the increased selective release of lysosomal acid hydrolases is not affected.
Cephalosporin antibiotics can be modified to inhibit human leukocyte elastase
It is reported that neutral cephalosporins (that is, compounds not bearing a free carboxyl at position C-4) can be modified to become potent time-dependent inhibitors of HLE.
The selective release of phospholipase A2 by resident mouse peritoneal macrophages.
This release is specifically elicited by an inflammatory substance such as zymosan, since macrophages incubated with 6 micrometer latex spheres retain pH-4.5 phospholipase A2 activity and lysosomal enzyme activities intracellularly.
Inhibitor of phospholipase A2 blocks eicosanoid and platelet activating factor biosynthesis and has topical anti-inflammatory activity.
- K. Tramposch, F. Chilton, R. Bonney
- Medicine, BiologyJournal of Pharmacology and Experimental…
- 1 November 1994
BMS-181162 is an inhibitor ofPLA2 and may prove to be a useful tool in the delineation of the role of PLA2 in the inflammatory process.
Primary monolayer cultures of adult rat liver parenchymal cells suitable for study of the regulation of enzyme synthesis
Parenchymal cells from adult rat liver which had been fully regenerated were isolated and cultured in nonproliferating monolayers in vitro andTyrosine aminotrasferase activity in the cells was elevated when the culture medium was supplemented with hydrocortisone or dexamethasone.
Adult liver parenchymal cells in primary culture: Characteristics and cell recognition standards
- R. Bonney
- BiologyIn Vitro
- 1 July 1974
The results of this study demonstrate that such a culture can perform many of the specific functions characteristic of the adult liver in vivo, and offers many applications for the study of the regulation of adult liver metabolism.
Antigen-antibody complexes stimulate the synthesis and release of prostaglandins by mouse peritoneal macrophages.
Physiological and Pharmacological Regulation of Prostaglandin and Leukotriene Production by Macrophages
The synthesis and secretion of prostaglandins and leukotrienes by mouse peritioneal macrophages is under several regulatory controls and can be regulated by certain antiinflammatory compounds.