• Publications
  • Influence
Mechanisms of sodium pump regulation.
  • A. Therien, R. Blostein
  • Chemistry, Medicine
  • American journal of physiology. Cell physiology
  • 1 September 2000
TLDR
An overview of the many mechanisms in place to regulate sodium pump activity in a tissue-specific manner is presented, including regulation by substrates, membrane-associated components such as cytoskeletal elements and the gamma-subunit, and circulating endogenous inhibitors as well as a variety of hormones. Expand
Tissue- and isoform-specific kinetic behavior of the Na,K-ATPase.
TLDR
The objective of this study has been to delineate the side-specific effects of Na+ and K+ on the transport kinetics of tissue-specific Na/K pumps and the functional differences observed with the individual isoform-transfected cells were completely consistent with the kinetic differences observedwith the axolemma and kidney pumps fused into erythrocytes. Expand
Tissue-specific Versus Isoform-specific Differences in Cation Activation Kinetics of the Na,K-ATPase (*)
TLDR
Tissue-specific differences in Na and K activation kinetics of Na,K-ATPase activity of the same species (rat) were observed when the same isoform was assayed in different tissues or cells, and a kinetic analysis of the apparent affinities for Na as a function of K concentration indicates that isoform-specific as well as tissue- specific differences are related to the apparentaffinities. Expand
Insulin induces translocation of the alpha 2 and beta 1 subunits of the Na+/K(+)-ATPase from intracellular compartments to the plasma membrane in mammalian skeletal muscle.
TLDR
It is suggested that insulin induces an isoform-specific translocation of Na+ pump subunits from different intracellular sources to the PM and that the hormone-responsive enzyme in rat skeletal muscle is an alpha 2:beta 1 dimer. Expand
Relationships between erythrocyte membrane phosphorylation and adenosine triphosphate hydrolysis.
  • R. Blostein
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 25 April 1968
TLDR
Human erythrocyte membranes were incubated with a low concentration of terminally labeled ATP with the aim of detecting a phosphorylated intermediate of adenosine triphosphatase activity, and it was shown that the approximate rate of turnover was similar to the rate of ATP hydrolysis. Expand
Tissue-specific Distribution and Modulatory Role of the γ Subunit of the Na,K-ATPase
TLDR
Evidence that the pig γ subunit, which migrates as a doublet on polyacrylamide gels, is sensitive to digestion by trypsin, and that Rb+ions partially protect it against this effect, indicate that the γSubunit is a tissue-specific regulator which shifts the steady-state equilibria toward E1. Expand
Functional Role and Immunocytochemical Localization of the γa and γb Forms of the Na,K-ATPase γ Subunit*
TLDR
The importance of γa and γb may be related to their partially overlapping but distinct expression patterns and tissue-specific functions of the pump that these serve. Expand
COMPARATIVE STUDIES OF LIVER AND MUSCLE ALDOLASE. II. IMMUNOCHEMICAL AND CHROMATOGRAPHIC DIFFERENTIATION.
TLDR
It is demonstrated that mammalian systems contain at least two distinct enzymatic entities exhibiting aldolase activity, one exhibiting a high selectivity for fructose diphosphate and a high turnover number, and the other, a broader substrate specificity and a lower catalytic activity. Expand
A functional interaction between CHIF and Na-K-ATPase: implication for regulation by FXYD proteins.
TLDR
Rb flux experiments demonstrate that CHIF induces a two- to threefold increase in apparent affinity for cytoplasmic Na but does not affect affinity for extracellular K (Rb) ions (K'(K)) or V(max). Expand
Kinetic Alterations due to a Missense Mutation in the Na,K-ATPase α2 Subunit Cause Familial Hemiplegic Migraine Type 2*
TLDR
It is suggested that the decreased apparent K+ affinity is the basis for a reduced rate of extracellular K+ removal, which delays the recovery phase of nerve impulse transmission in the central nervous system and, thereby, the clinical picture of migraine with aura. Expand
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