R. Bhatnagar

Publications147
h-index33
Citations4,833
Highly Influential Citations226
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RNA Interference: Biology, Mechanism, and Applications
TLDR
Because of its exquisite specificity and efficiency, RNAi is being considered as an important tool not only for functional genomics, but also for gene-specific therapeutic activities that target the mRNAs of disease-related genes.
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Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial…
TLDR
This is the first attempt to study the midgut microbiota of Anopheles stephensi from lab-reared and field-collected adult and larvae using "culture-dependent and independent methods", and several of the bacteria identified are related to the known symbionts in other insects.
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Silencing of Midgut Aminopeptidase N of Spodoptera litura by Double-stranded RNA Establishes Its Role asBacillus thuringiensis Toxin Receptor*
TLDR
Administration of slapn double-stranded RNA to S. litura larvae reduces its expression and this results directly implicate larval midgut aminopeptidase N as receptor for Bacillus thuringiensis insecticidal proteins.
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High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress
TLDR
In this procedure, about 33% of globular embryos not only developed into the cotyledonary stage but rooted simultaneously, eliminating a separate rooting step, and significant improvement in embryogenesis was achieved.
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Toxicity Analysis of N- and C-Terminus-Deleted Vegetative Insecticidal Protein from Bacillus thuringiensis
TLDR
Analysis of insecticidal activity with N- and C-terminus deletion mutants suggests a differential mode of action of VIP against different pests.
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Knockdown of Aminopeptidase-N from Helicoverpa armigera Larvae and in Transfected Sf21 Cells by RNA Interference Reveals Its Functional Interaction with Bacillus thuringiensis Insecticidal Protein…
Aminopeptidase-N (APN) and cadherin proteins located at the midgut epithelium of Helicoverpa armigera have been implicated as receptors for the Cry1A subfamily of insecticidal proteins of Bacillus…
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Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest
TLDR
The gene families encoding receptors for bitter or toxic substances and detoxification enzymes, such as cytochrome P450, carboxylesterase and glutathione-S-transferase were massively expanded in this polyphagous species, enabling its extraordinary ability to detect and detoxify many plant secondary compounds.
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Recombinantly expressed isoenzymic aminopeptidases from Helicoverpa armigera (American cotton bollworm) midgut display differential interaction with closely related Bacillus thuringiensis…
TLDR
This is the first report demonstrating differential Cry-toxin-binding abilities of two different aminopeptidases from a susceptible insect.
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A constitutively expressed 36 kDa exochitinase from Bacillus thuringiensis HD-1.
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Role of RNA Interference (RNAi) in Dengue Virus Replication and Identification of NS4B as an RNAi Suppressor
TLDR
The results suggest the involvement of miRNA/RNAi pathways in dengue virus establishment and that d Dengue virus NS4B protein plays an important role in the modulation of the host RNAi/miRNA pathway to favor dengued virus replication.
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