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Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
TLDR
It is shown that the JFH1 genome replicates efficiently and supports secretion of viral particles after transfection into a human hepatoma cell line (Huh7) and provides a powerful tool for studying the viral life cycle and developing antiviral strategies.
Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.
TLDR
This work defines the structure of HCV replicons functional in cell culture and provides the basis for a long-sought cellular system that should allow detailed molecular studies ofHCV and the development of antiviral drugs.
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
TLDR
Cardif is described, a new CARD-containing adaptor protein that interacts with RIG-I and recruits IKKα, IKKβ and IKKɛ kinases by means of its C-terminal region, leading to the activation of NF-κB and IRF3.
The lipid droplet is an important organelle for hepatitis C virus production
TLDR
A novel function of LDs is revealed in the assembly of infectious HCV and a new perspective on how viruses usurp cellular functions is provided.
Replication of hepatitis C virus.
TLDR
This review summarizes the current knowledge about HCV replication and describes attempts pursued in the last few years to establish efficient and reliable cell culture systems.
Enhancement of Hepatitis C Virus RNA Replication by Cell Culture-Adaptive Mutations
TLDR
Cell culture-adaptive mutations enhance HCV RNA replication and are corroborated with replicons in which the selectable marker gene was replaced by the gene encoding firefly luciferase.
Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Hepatitis C Virus Replication
TLDR
The morphology of the membranous rearrangements induced in HCV-infected cells resemble those of the unrelated picorna-, corona- and arteriviruses, but are clearly distinct from those from the closely related flavivirus.
Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras.
TLDR
A series of further chimeric genomes allowing production of infectious genotype (GT) 1a, 1b, 2a, and 3a particles are created, suggesting that determinants within the structural proteins govern kinetic and efficiency of virus assembly and release and an E1-specific antiserum capable of neutralizing infectivity of all HCV chimeras is described.
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