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Flap endonuclease 1: a central component of DNA metabolism.
TLDR
FEN1 is a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions and interacts with other nucleases and helicases that allow it to act efficiently on structured flaps.
Cleavage Specificity of Saccharomyces cerevisiae Flap Endonuclease 1 Suggests a Double-Flap Structure as the Cellular Substrate*
TLDR
Results suggest that the double-flap substrate is formed and cleaved during eukaryotic DNA replication in vivo.
Strand transfer events during HIV-1 reverse transcription.
TLDR
Viral nucleocapsid protein (NC) functions to promote transfer by facilitating this strand exchange process, and details of the role of strand transfers in reverse transcription and the biochemical features of the transfer reaction are discussed.
Macrophage Tropism of HIV-1 Depends on Efficient Cellular dNTP Utilization by Reverse Transcriptase*
TLDR
The results suggest that the ability of HIV-1 to infect macrophages depends, at least in part, on enzymatic adaptation ofAIDS-1 RT to efficiently catalyze DNA synthesis in limited cellular dNTP substrate environments.
Regulatory Roles of p21 and Apurinic/Apyrimidinic Endonuclease 1 in Base Excision Repair*
TLDR
It is shown that p21 also inhibits PCNA stimulation of long patch base excision repair (BER) in vitro and proposed that APE1 serves as an assembly and coordination factor for long patch BER proteins, which implies that BER can be regulated differentially.
Enzymes and Reactions at the Eukaryotic DNA Replication Fork*
TLDR
This work will focus on recent findings concerning specific enzymatic reactions necessary for the growth of the eukaryotic replication fork.
Ataxia Telangiectasia Mutated (ATM) and ATM and Rad3-related Protein Exhibit Selective Target Specificities in Response to Different Forms of DNA Damage*
TLDR
Activated ATM and ATR exhibit selective substrate specificity in response to different genotoxic agents as revealed by activation of these kinases in cells exposed to IR, UV, and hyperoxia.
Sequence Requirements for Estrogen Receptor Binding to Estrogen Response Elements*
TLDR
It is found that changes in the sequences flanking a nonconsensus ERE can greatly alter ER-ERE affinity, either positively or negatively, and rules that predict whether ER binds to a given natural ERE are developed.
Enzymatic completion of mammalian lagging-strand DNA replication.
TLDR
Using purified proteins from calf and a synthetic substrate, the enzymatic reactions required for mammalian Okazaki fragment processing in vitro are reconstituted and strongly suggest that they perform the same reactions in vivo.
Anti-HIV Host Factor SAMHD1 Regulates Viral Sensitivity to Nucleoside Reverse Transcriptase Inhibitors via Modulation of Cellular Deoxyribonucleoside Triphosphate (dNTP) Levels*
TLDR
It is found that reduction of SAMHD1 levels with the use of virus-like particles expressing Vpx- and SAM HD1-specific shRNA subsequently elevates cellular dNTPs and significantly decreases HIV-1 sensitivity to various NRTIs in macrophages.
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