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Cross-linking of protein by ω-maleimido alkanoylN-hydroxysuccinimido esters
A series of chemical bifunctional cross-linking reagents, the succinimidyl maleimides, has been synthesized. Using hemoglobin as a model protein, it has been shown that these molecules react rapidly
Interactions between the mitochondrial adenosinetriphosphatase and periodate-oxidized adenosine 5'-triphosphate, an affinity label for adenosine 5'-triphosphate binding sites.
The nature and mechanism of the inhibition of ATPase activity exerted by o-ATP and the elimination product were examined and the significance of the beta-elimination reaction to the use of periodate-oxidized nucleotides as affinity labels of nucleotide binding sites on other proteins is discussed.
Mechanisms of phosphate transport in sheep intestine and parotid gland: response to variation in dietary phosphate supply
In sheep the gut appears to play a major role in response to phosphate deprivation, by increasing the capacity to transport phosphate, which is not achieved by increases in the levels of circulating 1,25‐dihydroxycholecalciferol.
Glycyl-L-proline transport in rabbit enterocyte basolateral-membrane vesicles.
The properties of a peptide-transport system in rabbit enterocyte basolateral membrane were examined with glycyl-L-proline as the substrate and the orientation of the basol lateral-membrane vesicles was shown to be predominantly inside-out on determination by two independent criteria.
Phosphate transport in intestinal brush-border membrane
It is concluded that the mechanism of the phosphate carrier is electrogenic at pH 6–8, probably two Na+ moving with each H2PO4−, which will lead to the development of a positive charge within the vesicle.
Escherichia coli mutants resistant to uncouplers of oxidative phosphorylation.
Two mutant strains of Escherichia coli resistant to the uncoupling agents 4,5,6,7-tetrachloro-2-trifluoromethyl benzimidazole and carbonyl cyanide m-chlorophenylhydrazone isolated are concluded to have arisen as a consequence of mutations which directly affect a specific site of uncoupler action within the cytoplasmic membrane.
A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles.
An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform and analyses on polyacrylamide gels and by electron microscopy show that it is similar to other ATPase preparations from bovines heart mitochondria.