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A simple purification of wheat germ agglutinin from commercial wheat germ is described. From defatted ground wheat germ, the lectin was extracted and then purified in a single step by filtration on an ion exchange chromatography column and adsorption on an insolubilized N-acetyl glucosamine derivative. The amount of lectin obtained from 1,000 g of wheat(More)
Streptococcus species are important causes of infective endocarditis but species identification remains challenging. We report two cases of infective endocarditis due to Streptococcus tigurinus-like organisms, which were first identified by 16S ribosomal RNA gene sequence analysis and subsequently confirmed using phylogeny based on the analysis of the shetA(More)
A study of the physical properties of paraprotein required until now, the use of fairly complicated techniques, such as analytical ultracentrifugation. The use of chromatography on acrylamide agar pearls and electrophoresis on indubiose plates, coupled with the classical techniques of electrophoresis and immunoelectrophoresis, permitted a more simple(More)