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Using cerebellar, neuron-enriched primary cultures, we have studied the glutamate receptor subtypes coupled to neurotransmitter amino acid release. Acute exposure of the cultures to micromolar concentrations of kainate and quisqualate stimulated D-[3H]aspartate release, whereas N-methyl-D-aspartate, as well as dihydrokainic acid, were ineffective. The(More)
Micromolar concentrations of kainic acid and quisqualic acid released [3H]D-aspartate preaccumulated by cerebellar granule cells in culture. The effect of kainate was selectively antagonized by kynurenic acid and, less effectively, by PDA. Kainate and quisqualate also increased [3H]GABA release from a subpopulation of cultured cerebellar astrocytes.(More)
We have used postnatal rat cerebellar astrocyte-enriched cultures to study the excitatory amino acid receptors present on these cells. In the cultures used, type-2 astrocytes (recognized by the monoclonal antibodies A2B5 and LB1) selectively took up gamma-[3H]aminobutyric acid ([3H]GABA) and released it when incubated in the presence of micromolar(More)
The effect of acute exposure to micromolar concentrations of kainic acid was studied in rat cerebellar astroglial cultures consisting of two antigenically and functionally distinct subpopulations of astrocytes. Kainate (20-100 microM) released [3H]GABA preaccumulated by A2B5-positive stellate astrocytes in a concentration-, Na+- and Ca2+-dependent way, and(More)
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