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We investigated the growth of islet beta and alpha cells in adult rats which had undergone partial pancreatic duct ligation. Whereas the non-ligated head portion of the pancreas remained unaffected in terms of histology and cell population dynamics, the ligated tail part of the pancreas showed pronounced changes in histology and cell growth. These changes(More)
Cytokeratins (CKs) serve as immunocytochemical markers of epithelial cells. We found that duct cells of the neonatal and adult rat pancreas express CKs 7, 19, and 20. Because pancreatic endocrine cells are thought to derive from duct cells, we examined their expression of CKs 7, 19, and 20 during the neonatal period and the proliferative activity of the(More)
Islet transplantation is associated with a high rate of early graft failure, a problem that remains poorly understood. It is probable that the destruction of the islet microenvironment and loss of tropic support that occur during isolation lead to compromised survival. The purpose of this study was to determine the role of matrix-integrin interactions on(More)
Regeneration of neonatal beta cells after streptozotocin (STZ)-induced destruction may be due to either replication from pre-existing intra-islet beta cells or extra-islet precursor cells. To further investigate this issue, beta-cell growth was analysed in normal and streptozotocin-treated newborn rats (100 μg/g body weight) at several time points during(More)
The purpose of this study was to characterize the trophic effect of pancreatic duct cells on the islets of Langerhans. Ductal epithelium and islets were isolated from hamster pancreata. In addition, duct-conditioned medium (DCM) was prepared from primary duct cultures that had been passaged twice to remove other cellular elements. Three experimental groups(More)
Regeneration of neonatal beta cells after subtotal streptozotocin (STZ)-induced destruction is incomplete but nevertheless leads to rapid remission of hyperglycaemia. To study the proliferative and functional capacity of regenerated beta cells in adolescent and adult rats after early beta-cell damage and to determine the time point after birth which is(More)
The reasons for the failure of clinical islet transplantation remain obscure. Islet isolation, however, exposes the islet to variety of cellular stresses, including disruption of the cell-matrix relationship, an event associated with apoptosis. The cell-matrix relationship is characterized by an interaction between cell surface integrin receptors and matrix(More)
In adult rats islet cell neogenesis can be stimulated by partial duct ligation. Duct to islet cell differentiation is thought to be regulated by growth factors such as gastrin and transforming growth factor-α (TGFα). To test this hypothesis, we examined the expression of gastrin and TGFα at the mRNA and protein level in pancreatic tissue following partial(More)
Ethyl tertiary-butyl ether (ETBE) is a motor fuel oxygenate used in reformulated gasoline. The current use of ETBE in gasoline or petrol is modest but increasing. To investigate the effects of ETBE on splenocytes, mice were exposed to 0 (control), 500 ppm, 1750 ppm, or 5000 ppm of ETBE by inhalation for 6 h/day for 5 days/wk over a 6- or 13-week period.(More)
Nanostructured Ni(OH)2 thin films were prepared by a simple solution growth process with F(-) and NH3 used as Ni2+ coordination agents, and ammonia hydroxide solution used as OH(-) supplier to accelerate the hydrolyzation of nickel complex species. The results showed Ni(OH)2 thin films were constructed mainly with hexagonal beta-Ni(OH)2 nanorods; the F(-)(More)
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