R S Kincade

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Membrane-associated folate (pteroylglutamate, PteGlu)-binding proteins (FBPs) play an important role as PteGlu-transport proteins in malignant and normal human cells. Since high extracellular folate (PteGlu) concentrations (EFC) profoundly influenced uptake and toxicity of the anti-PteGlu methotrexate in malignant KB cells, we studied human cells to(More)
  • A C Antony, R S Kincade, R S Verma, S R Krishnan
  • The Journal of clinical investigation
  • 1987
Mature human erythrocyte membranes contained specific, high affinity (Kd 3.3 X 10(-11) M) folate binding moieties. Folate binding was pH, time- and temperature-dependent, saturable, and was much greater for pteroylmonoglutamate and 5-methyltetrahydrofolate than 5-formyltetrahydrofolate and amethopterin. On detergent solubilization of membranes, two peaks of(More)
High-affinity membrane-associated and soluble folate binding proteins (FBPs) from human placenta, milk, and KB cells appear to share antigenic determinants [A. C. Antony et al. (1981) J. Biol. Chem. 256, 9684-9692 and (1985) 260, 14911-14917]. Iodination of a highly purified preparation of placental folate receptor (PFR) by various techniques resulted in(More)
A simplified method of screening for antisperm antibodies has been previously described using rabbit antihuman immunoglobulin immunobeads (GAM beads). These reports have indicated a high correlation between the GAM bead and maximal individual isotype binding. However, preliminary data in our laboratory (using a 14% cut-off criterion) indicated a high(More)
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