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The identification of Gram-negative pathogenic and non-pathogenic bacteria commonly isolated from an orchard phylloplane may result in a time consuming and tedious process for the plant pathologist. The paper provides a simple "one-step" protocol that uses the polymerase chain reaction (PCR) to amplify intergenic spacer regions between 16S and 23S genes and(More)
Restriction fragment length polymorphisms from PCR amplified ribosomal DNAs of three Trichogramma species, T. minutum, T. brassicae, and T. near sibiricum, were studied. Length variation in the internal transcribed spacer (ITS) region was observed. The ITS region of T. brassicae is about 1350 base pairs (bp) in length and those of T. minutum and T. near(More)
Mansonones A and C to G, sesquiterpene quinones possessing antifungal properties, were isolated from the elmUlmus americana infected withCeratocystis ulmi. Scopoletin and β-sitosterol were also present in the extracts. The mansonones were not detected on chromatographic analysis of extracts from healthy elm tissue. The extent to which these antifungal(More)
The natural population structure of the Dutch elm pathogen Ophiostoma ulmi was determined from isolated collected from across a Western Canadian disease front through an analysis of restriction-site polymorphisms in the ribosomal DNA repeat, length mutations in the mitochondrial genomes, and through DNA fingerprinting of the nuclear genomes using a(More)
Eighteen isolates representing six Fusarium species from diverse hosts and geographical origins were evaluated to determine ribosomal DNA variation using polymerase chain reaction and restriction fragment length polymorphisms. No length variation was observed for amplified 18S and 28S regions. However, amplification of the ITS region showed one isolate, a(More)
This study examined the polymerase chain reaction (PCR) amplified internal transcribed spacer (ITS) and 26S ribosomal DNA (rDNA) regions of 15 entomopathogenic nematode isolates including Steinernema feltiae syn. bibionis, S. glaseri, seven strains of S. carpocapsae, four strains of Heterorhabditis bacteriophora, and two field isolates. RDNA length(More)
The accumulation of mansonones E and F was investigated in Ulmus americana L. seedlings 5 weeks after inoculation with three aggressive and three non-aggressive isolates of Ophiostoma ulmi (Buism.) Nannf. The three non-aggressive isolates stimulated significantly more mansonone E and F accumulation than the three aggressive isolates of O. ulmi. Mansonone(More)
We aim to gain a fundamental understanding of how to achieve low friction and wear in ultrastrong carbon-based materials. Experimentally, we use an in situ nanotribometry method, which enables nanoscale visualization of sliding contacts inside the transmission electron microscope (TEM). These experiments are in turn modelled computationally using molecular(More)
Eighteen isolates representing six Fusarium species from diverse hosts and geographical origins were evaluated to determine ribosomal DNA variation using polymerase chain reaction and restriction fragment length polymorphisms. No length variation was observed for amplified 18S and 28S regions. However, amplification of the ITS region showed one isolate, a(More)
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