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We have identified a family of abundant peripheral plasma membrane glycoproteins that is unique to flowering plants. They are identified by a monoclonal antibody, MAC 207, that recognizes an epitope containing L-arabinose and D-glucuronic acid. Immunofluorescence and immunogold labeling studies locate the MAC 207 epitope to the outer surface of the plasma(More)
1. The use of a modified sodium chlorite/acetic acid delignification procedure for the solubilization of a hydroxyproline-rich glycoprotein fraction from the depectinated cell walls of Phaseolus coccineus is described. 2. The crude glycoprotein was associated with some pectic material; hydroxyproline and serine were the most abundant amino acids, and(More)
Improved methods of isolation and analysis of cell walls from a range of plant tissues have shed new light on the structure of the constituent polymers, and have also helped to clarify some of the conflicting opinions on their mode of occurrence and association within the walls. The chemistry (and biochemistry) of pectic and hemicellulosic polymers in(More)
1. Methylation analysis of potato (Solanum tuberosum) lectin and thorn-apple (Datura stramonium) lectin confirmed previous conclusions that both glycoproteins contained high proportions of l-arabinofuranosides and lesser amounts of d-galactopyranosides. The arabinofuranosides are present in both lectins as short unbranched chains containing 1-->2- and(More)
Cell-wall material of wheat bran was incubated with human faecal bacteria for 24-72 h and the resulting structural changes were studied by methylation analysis. Of the carbohydrate content, approximately 39% was degraded after 24 h, increasing to only 44% after 72 h. Arabinoxylans and mixed-linkage beta-D-glucans from the aleurone layer were degraded(More)